LASP1 Gene (LIM and SH3 Protein 1)
Actin-binding scaffold protein involved in cell migration, proliferation, and cancer progression
Gene Information Card
| Symbol | LASP1 |
|---|---|
| Full Name | LIM and SH3 protein 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 17q12 |
| NCBI Gene ID | 3927 ncbi.nlm.nih.gov/gene/3927 |
| Ensembl ID | ENSG00000002834 |
| UniProt ID | Q14847 |
| OMIM ID | 602920 |
| HGNC ID | 6513 |
| Aliases | MLN50, LASP-1 |
Description
LASP1 (LIM and SH3 protein 1) is a gene encoding a 261-amino acid actin-binding scaffold protein. It contains an N-terminal LIM domain and a C-terminal SH3 domain, enabling interactions with cytoskeletal components and signaling molecules. LASP1 is involved in cell migration, proliferation, and survival, and is overexpressed in multiple cancers, including breast, colorectal, and ovarian carcinomas. Its expression is regulated by transcription factors such as ETS1 and MYC.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression promotes cell migration and invasion via actin cytoskeleton remodeling and focal adhesion turnover. | PMID: 21573173; COSMIC overexpression data |
| Colorectal cancer | Upregulation correlates with lymph node metastasis and poor prognosis; LASP1 enhances Wnt/β-catenin signaling. | PMID: 24986688; ClinVar somatic variants |
| Ovarian cancer | Increased expression associated with tumor progression and chemoresistance through PI3K/AKT pathway activation. | PMID: 25964277; COSMIC mutation frequency |
| Hepatocellular carcinoma | LASP1 knockdown reduces proliferation and invasion; acts via FAK/Src signaling. | PMID: 27667693 |
| Gastric cancer | High expression linked to advanced stage and shorter survival; promotes epithelial-mesenchymal transition. | PMID: 28289123 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Breast | 12.5 nTPM | Medium |
| Colon | 10.2 nTPM | Medium |
| Ovary | 8.9 nTPM | Low |
| Liver | 6.7 nTPM | Low |
| Stomach | 9.1 nTPM | Low |
| Lung | 7.4 nTPM | Low |
| Kidney | 5.3 nTPM | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 15.8 nTPM | High expression; promotes migration |
| HCT116 (colorectal cancer) | 13.2 nTPM | High expression; linked to metastasis |
| SKOV3 (ovarian cancer) | 11.5 nTPM | Moderate expression; chemoresistance |
| HepG2 (hepatocellular carcinoma) | 9.7 nTPM | Moderate expression; invasion |
| A549 (lung cancer) | 6.4 nTPM | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.487C>T (p.Arg163Trp) | Missense | <0.1% in COSMIC | Unknown; predicted to affect SH3 domain binding |
| c.124G>A (p.Glu42Lys) | Missense | <0.1% in COSMIC | Unknown; located in LIM domain |
| c.1-?_*?del | Frameshift/deletion | Rare in ClinVar | Loss of function; not associated with disease |
Mutation functional classification
Loss of Function (LOF)
No established loss-of-function mutations; rare deletions may reduce protein expression but no disease phenotype reported.
Gain of Function (GOF)
Overexpression (not mutation) is the primary gain-of-function mechanism in cancer; no activating mutations described.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Focal adhesion (KEGG: hsa04510)
• Actin cytoskeleton regulation (KEGG: hsa04810)
• PI3K-Akt signaling pathway (KEGG: hsa04151)
• Wnt signaling pathway (KEGG: hsa04310)
Protein Summary
LASP1 is a 30 kDa scaffold protein with a LIM domain (residues 2-60) and an SH3 domain (residues 200-261). It localizes to focal adhesions, lamellipodia, and the nucleus. Through its LIM domain, it binds to zyxin and other focal adhesion proteins; the SH3 domain interacts with kinases such as Src and FAK. LASP1 regulates actin dynamics, cell adhesion, and signal transduction. Overexpression in cancers correlates with metastasis and poor prognosis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| LASP1 Knockout HEK293 Cell Line | EDJ-KQ4331 | Human | 3927 | Details Get a Quote |
| LASP1NB Knockout HEK293 Cell Line | EDJ-KQ14028 | Human | 100505576 | Details Get a Quote |
| LASP1 Knockout A-549 Cell Line | EDJ-KQ28059 | Human | 3927 | Details Get a Quote |
| LASP1 Knockout HCT 116 Cell Line | EDJ-KQ28060 | Human | 3927 | Details Get a Quote |
| LASP1 Knockout HeLa Cell Line | EDJ-KQ28061 | Human | 3927 | Details Get a Quote |
| LASP1NB Knockout A-549 Cell Line | EDJ-KQ43942 | Human | 100505576 | Details Get a Quote |
| LASP1NB Knockout HCT 116 Cell Line | EDJ-KQ43943 | Human | 100505576 | Details Get a Quote |
| LASP1NB Knockout HeLa Cell Line | EDJ-KQ43944 | Human | 100505576 | Details Get a Quote |
