KLHL32 Gene: Kelch Like Family Member 32 | NCBI, UniProt, OMIM

KLHL32 (Kelch Like Family Member 32) is a protein-coding gene located on chromosome 6q21. It encodes a member of the BTB-Kelch protein family, characterized by an N-terminal BTB/POZ domain and C-terminal Kelch repeats. These proteins typically function as substrate-specific adaptors for CUL3-based E3 ubiquitin ligases, targeting proteins for ubiquitination and proteasomal degradation. KLHL32 is involved in cellular processes including cytoskeletal organization, cell cycle regulation, and signal transduction. Expression is observed in multiple tissues, with notable levels in the brain and testis.

Tissue Expression

Cell Line Expression

Hotspot Mutations

Mutation functional classification

• Ubiquitin mediated proteolysis (KEGG: hsa04120)

• CUL3-RING ubiquitin ligase complex

KLHL32 is a 627-amino acid protein containing an N-terminal BTB/POZ domain (residues 40-140) and six C-terminal Kelch repeats (residues 280-620). The BTB domain mediates homodimerization and interaction with CUL3, while the Kelch repeats form a beta-propeller structure that recognizes specific substrates. KLHL32 is predicted to function as a substrate adaptor for CUL3-based E3 ubiquitin ligases, targeting proteins for ubiquitination. Structural modeling suggests the Kelch domain forms a conserved binding pocket for substrate recognition. Post-translational modifications include phosphorylation sites (e.g., Ser200, Thr450) that may regulate activity.