KLF8: Kruppel-like Factor 8 – Transcriptional Regulator in Cancer and Development
Comprehensive biomedical overview of KLF8 gene, including genomic context, expression, mutations, and disease associations.
Gene Information Card
| Symbol | KLF8 |
|---|---|
| Full Name | Kruppel-like factor 8 |
| Gene Type | protein-coding |
| Chromosomal Location | Xp11.21 |
| NCBI Gene ID | 11279 ncbi.nlm.nih.gov/gene/11279 |
| Ensembl ID | ENSG00000102359 |
| UniProt ID | O95600 |
| OMIM ID | 300286 |
| HGNC ID | 6349 |
| Aliases | BKLF3, ZNF741 |
Description
KLF8 (Kruppel-like factor 8) is a member of the Kruppel-like factor (KLF) family of transcription factors, characterized by three C-terminal C2H2 zinc finger domains. It binds to GC-rich sequences in gene promoters and regulates transcription of genes involved in cell proliferation, differentiation, and oncogenesis. KLF8 is implicated in epithelial-mesenchymal transition (EMT) and cancer metastasis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | KLF8 overexpression promotes EMT and invasion via transcriptional activation of MMP9 and Snail. | PMID: 23454746; NCBI GeneRIF |
| Breast cancer | KLF8 upregulation correlates with poor prognosis; enhances cell cycle progression and metastasis. | PMID: 20010871; ClinVar |
| Gastric cancer | KLF8 activates Wnt/β-catenin signaling, contributing to tumor growth and invasion. | PMID: 26014226; COSMIC |
| Ovarian cancer | KLF8 overexpression linked to chemoresistance and aggressive phenotype. | PMID: 24130169; UniProt |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 15.2 | Medium |
| Placenta | 8.7 | Low |
| Liver | 4.1 | Low |
| Breast | 3.5 | Low |
| Ovary | 2.9 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 (liver cancer) | 12.3 | Highest expression in liver cancer lines |
| MCF7 (breast cancer) | 8.1 | Moderate expression |
| A549 (lung cancer) | 5.4 | Low expression |
| K562 (leukemia) | 2.1 | Very low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1012C>T (p.Arg338Trp) | Missense | <0.01% | Unknown functional effect; reported in COSMIC |
| c.784G>A (p.Gly262Ser) | Missense | <0.01% | Located in zinc finger domain; potential loss of DNA binding |
| c.1234_1235insA | Frameshift | <0.01% | Predicted loss of function; truncation |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations in the zinc finger domain are predicted to abolish DNA binding and transcriptional activity.
Gain of Function (GOF)
Missense mutations in the N-terminal domain may enhance transactivation; not well characterized.
Dominant Negative (DN)
No confirmed dominant-negative mutations reported in KLF8.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Epithelial-to-mesenchymal transition (EMT) – KLF8 activates Snail and MMP9
• Wnt/β-catenin signaling – KLF8 enhances β-catenin/TCF transcriptional activity
• Cell cycle – KLF8 regulates cyclin D1 and p21 expression
Protein Summary
KLF8 is a 359-amino-acid transcription factor with three C2H2 zinc fingers at the C-terminus. It localizes to the nucleus and binds GC-rich promoter elements. KLF8 can act as both a transcriptional activator and repressor, depending on context. It is involved in cell cycle control, EMT, and cancer progression. Post-translational modifications include phosphorylation and sumoylation, which modulate its activity.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| KLF8 Knockout HEK293 Cell Line | EDJ-KQ1101 | Human | 11279 | Details Get a Quote |
| KLF8 Knockout HeLa Cell Line | EDJ-KQ55623 | Human | 11279 | Details Get a Quote |
| KLF8 Knockout A-549 Cell Line | EDJ-KQ64122 | Human | 11279 | Details Get a Quote |
| KLF8 Knockout HCT 116 Cell Line | EDJ-KQ72566 | Human | 11279 | Details Get a Quote |
Displaying Records 1 To 4 Of 4 Records
