KIFC1
Kinesin Family Member C1, a minus-end-directed microtubule motor protein involved in centrosome clustering and mitotic spindle assembly
Gene Information Card
| Symbol | KIFC1 |
|---|---|
| Full Name | kinesin family member C1 |
| Gene Type | protein-coding |
| Chromosomal Location | 6p21.32 |
| NCBI Gene ID | 3833 ncbi.nlm.nih.gov/gene/3833 |
| Ensembl ID | ENSG00000112742 |
| UniProt ID | Q9BW19 |
| OMIM ID | 603763 |
| HGNC ID | 6389 |
| Aliases | HSET, KNSL2, KIFC1A |
Description
KIFC1 (kinesin family member C1) encodes a minus-end-directed kinesin motor protein that plays a critical role in centrosome clustering, bipolar spindle assembly, and chromosome segregation during mitosis. It is overexpressed in various cancers and is considered a potential therapeutic target due to its essential function in cancer cells with supernumerary centrosomes.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression of KIFC1 promotes centrosome clustering, enabling survival of aneuploid cells | PMID: 24755471 |
| Ovarian cancer | KIFC1 upregulation correlates with poor prognosis and resistance to chemotherapy | PMID: 29367642 |
| Glioblastoma | KIFC1 is required for mitotic fidelity in glioblastoma stem-like cells | PMID: 31085178 |
| Lung adenocarcinoma | KIFC1 overexpression associated with tumor progression and metastasis | PMID: 31591465 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Bone marrow | 8.3 | Low |
| Lymph node | 6.1 | Low |
| Brain | 4.2 | Low |
| Breast | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.2 | Cervical cancer cell line; high expression |
| MCF7 | 12.8 | Breast cancer cell line; moderate expression |
| A549 | 10.5 | Lung cancer cell line; moderate expression |
| U2OS | 14.1 | Osteosarcoma cell line; high expression |
| HEK293 | 7.3 | Embryonic kidney cell line; low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1045C>T (p.Arg349Trp) | Missense | <0.01% (gnomAD) | Unknown functional effect; not reported in COSMIC |
| c.1720G>A (p.Glu574Lys) | Missense | <0.01% (gnomAD) | Unknown functional effect; not reported in COSMIC |
| c.1960_1961insA (p.Thr654AsnfsTer2) | Frameshift | <0.01% (gnomAD) | Predicted loss of function; not reported in COSMIC |
Mutation functional classification
Loss of Function (LOF)
Frameshift insertion c.1960_1961insA leads to premature stop codon, likely resulting in nonsense-mediated decay or truncated non-functional protein.
Gain of Function (GOF)
No gain-of-function mutations have been reported in KIFC1.
Dominant Negative (DN)
No dominant-negative mutations have been characterized for KIFC1.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Kinesins (Reactome: R-HSA-983189)
• Mitotic Prometaphase (Reactome: R-HSA-68877)
• Resolution of Sister Chromatid Cohesion (Reactome: R-HSA-2500257)
Protein Summary
KIFC1 is a 673-amino-acid protein with a kinesin motor domain at the N-terminus that moves along microtubules toward the minus end. It localizes to centrosomes and the mitotic spindle, where it crosslinks and slides microtubules to facilitate centrosome clustering and bipolar spindle formation. KIFC1 is essential for the viability of cancer cells with extra centrosomes, making it a target for anti-cancer therapies.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| KIFC1 Knockout HEK293 Cell Line | EDJ-KQ3077 | Human | 3833 | Details Get a Quote |
| KIFC1 Knockout A-549 Cell Line | EDJ-KQ22988 | Human | 3833 | Details Get a Quote |
| KIFC1 Knockout HCT 116 Cell Line | EDJ-KQ24367 | Human | 3833 | Details Get a Quote |
| KIFC1 Knockout HeLa Cell Line | EDJ-KQ24368 | Human | 3833 | Details Get a Quote |
