H3C10
Histone H3.1, a core component of nucleosomes involved in chromatin structure and gene regulation
Gene Information Card
| Symbol | H3C10 |
|---|---|
| Full Name | H3 clustered histone 10 |
| Gene Type | protein-coding |
| Chromosomal Location | 6p22.1 |
| NCBI Gene ID | 8358 ncbi.nlm.nih.gov/gene/8358 |
| Ensembl ID | ENSG00000277734 |
| UniProt ID | P68431 |
| OMIM ID | 602810 |
| HGNC ID | 4764 |
| Aliases | H3.1, H3C10, HIST1H3A, H3FA |
Description
H3C10 encodes histone H3.1, a core histone protein that forms the nucleosome with histones H2A, H2B, and H4. Histone H3.1 is a replication-dependent variant expressed during S phase and is essential for chromatin packaging, DNA replication, and transcriptional regulation. Post-translational modifications of H3.1, such as methylation and acetylation, regulate gene expression and chromatin state.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Pediatric glioblastoma | Recurrent H3F3A (H3.3) mutations are common; H3C10 (H3.1) mutations are rare but reported in diffuse intrinsic pontine glioma (DIPG) with K27M substitution | PMID: 22286216 |
| Diffuse intrinsic pontine glioma (DIPG) | H3.1 K27M mutation alters histone methylation and gene expression | PMID: 22286216 |
| Breast cancer | Overexpression and altered H3.1 modification patterns linked to tumor progression | PMID: 25686104 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 45.2 | High |
| Bone marrow | 38.7 | High |
| Lymph node | 32.1 | High |
| Brain | 12.5 | Medium |
| Liver | 8.3 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 52.3 | Cervical cancer cell line |
| K562 | 48.9 | Leukemia cell line |
| HEK293 | 41.5 | Embryonic kidney cell line |
| MCF7 | 35.2 | Breast cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| K27M | Missense | Rare in H3C10; common in H3F3A | Alters H3K27me3, drives oncogenesis in DIPG |
| G34R/V | Missense | Rare | Associated with pediatric glioblastoma |
Mutation functional classification
Loss of Function (LOF)
Not well characterized; H3.1 loss is lethal in cells
Gain of Function (GOF)
K27M acts as a dominant inhibitor of PRC2, reducing H3K27me3
Dominant Negative (DN)
K27M mutation disrupts normal histone modification patterns
View complete mutation data：
Gene Ontology (GO)
| • nucleosome assembly (GO:0006334) | • chromatin silencing (GO:0006342) |
| • DNA replication-dependent nucleosome assembly (GO:0006335) | • protein heterodimerization activity (GO:0046982) |
| • DNA binding (GO:0003677) |
Pathways
• Chromatin organization (Reactome: R-HSA-4839726)
• Packaging Of Telomere Ends (Reactome: R-HSA-171306)
• HDACs deacetylate histones (Reactome: R-HSA-3214815)
Protein Summary
Histone H3.1 (UniProt P68431) is a 136-amino acid core histone protein that forms the nucleosome octamer. It is highly conserved and undergoes numerous post-translational modifications including acetylation, methylation, and phosphorylation. H3.1 is replication-dependent and expressed during S phase. Mutations at lysine 27 (K27M) are oncogenic in pediatric gliomas.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| H3C10 Knockout HEK293 Cell Line | EDJ-KQ6209 | Human | 8357 | Details Get a Quote |
| H3C10 Knockout A-549 Cell Line | EDJ-KQ30046 | Human | 8357 | Details Get a Quote |
| H3C10 Knockout HCT 116 Cell Line | EDJ-KQ30047 | Human | 8357 | Details Get a Quote |
| H3C10 Knockout HeLa Cell Line | EDJ-KQ30048 | Human | 8357 | Details Get a Quote |
