FUT5 Gene - Fucosyltransferase 5
Comprehensive genomic and functional analysis of the FUT5 gene encoding alpha-(1,3)-fucosyltransferase 5, involved in Lewis antigen synthesis and selectin ligand formation.
Gene Information Card
| Symbol | FUT5 |
|---|---|
| Full Name | Fucosyltransferase 5 |
| Gene Type | protein-coding |
| Chromosomal Location | 19q13.33 |
| NCBI Gene ID | 2527 ncbi.nlm.nih.gov/gene/2527 |
| Ensembl ID | ENSG00000130309 |
| UniProt ID | Q11128 |
| OMIM ID | 136835 |
| HGNC ID | 4016 |
| Aliases | Fuc-T V, Fucosyltransferase V, Galactoside 3-L-fucosyltransferase, Lewis alpha-(1,3/1,4)-fucosyltransferase |
Description
FUT5 encodes alpha-(1,3)-fucosyltransferase 5, a Golgi membrane-bound enzyme that catalyzes the transfer of fucose from GDP-fucose to N-acetyllactosamine-type oligosaccharides, forming Lewis a (Lea) and Lewis x (Lex) antigens. These carbohydrate structures are critical for cell-cell adhesion, immune recognition, and pathogen binding. FUT5 is part of the fucosyltransferase family and is expressed in various tissues, with highest levels in the gastrointestinal tract and kidney.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal Cancer | FUT5 overexpression increases sialyl Lewis x/a antigens, promoting tumor cell adhesion to endothelial selectins and facilitating metastasis. | PMID: 12477932 |
| Helicobacter pylori Infection | FUT5-mediated Lewis b antigen expression on gastric epithelium enables H. pylori adhesion via BabA adhesin. | PMID: 10570172 |
| Inflammatory Bowel Disease | Altered FUT5 expression affects fucosylation of intestinal mucins, modulating gut microbiota and inflammation. | PMID: 27102614 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Colon | 12.5 | Medium |
| Small Intestine | 10.8 | Medium |
| Kidney | 8.2 | Medium |
| Stomach | 6.1 | Low |
| Liver | 2.3 | Low |
| Lung | 1.5 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HT-29 (Colorectal Adenocarcinoma) | 15.2 | High expression; used in fucosylation studies |
| Caco-2 (Colorectal Adenocarcinoma) | 11.0 | Moderate expression; enterocyte model |
| HEK 293 (Embryonic Kidney) | 4.5 | Low endogenous expression; common for recombinant FUT5 |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.511G>A (p.Ala171Thr) | Missense | <0.01% (gnomAD) | Unknown functional impact; predicted benign by SIFT |
| c.1043C>T (p.Thr348Met) | Missense | <0.01% (gnomAD) | Unknown functional impact; predicted tolerated |
| c.1246G>A (p.Gly416Ser) | Missense | <0.01% (gnomAD) | Unknown functional impact; predicted benign |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in FUT5.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in FUT5.
Dominant Negative (DN)
No dominant-negative mutations reported in FUT5.
View complete mutation data：
Gene Ontology (GO)
| • Golgi apparatus (GO:0005794) | • integral component of membrane (GO:0016021) |
| • alpha-(1,3)-fucosyltransferase activity (GO:0003829) | • protein N-linked glycosylation (GO:0006487) |
| • protein N-linked glycosylation via asparagine (GO:0016262) | • carbohydrate metabolic process (GO:0005975) |
Pathways
• ['Glycosphingolipid biosynthesis - lacto and neolacto series (KEGG: hsa00601)']
• ['Fucosylation of selectin ligands (Reactome: R-HSA-4085001)']
• ['Blood group glycolipid biosynthesis (Reactome: R-HSA-9033807)']
Protein Summary
Fucosyltransferase 5 (FUT5) is a 374-amino acid type II transmembrane protein localized to the Golgi apparatus. It catalyzes the addition of fucose in alpha-1,3 linkage to type 2 acceptor substrates (e.g., N-acetyllactosamine) and alpha-1,4 linkage to type 1 acceptors, producing Lewis x and Lewis a antigens, respectively. The protein contains a short N-terminal cytoplasmic tail, a transmembrane domain, and a large C-terminal catalytic domain facing the Golgi lumen. FUT5 shares high sequence homology with FUT3 and FUT6, and its activity is essential for the synthesis of sialyl Lewis epitopes involved in leukocyte trafficking and cancer metastasis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| FUT5 Knockout HEK293 Cell Line | EDJ-KQ50285 | Human | 2527 | Details Get a Quote |
| FUT5 Knockout HeLa Cell Line | EDJ-KQ53278 | Human | 2527 | Details Get a Quote |
| FUT5 Knockout A-549 Cell Line | EDJ-KQ61760 | Human | 2527 | Details Get a Quote |
| FUT5 Knockout HCT 116 Cell Line | EDJ-KQ70242 | Human | 2527 | Details Get a Quote |
