EIF3F
Eukaryotic Translation Initiation Factor 3 Subunit F
Gene Information Card
| Symbol | EIF3F |
|---|---|
| Full Name | eukaryotic translation initiation factor 3 subunit F |
| Gene Type | protein-coding |
| Chromosomal Location | 11p15.4 |
| NCBI Gene ID | 8665 ncbi.nlm.nih.gov/gene/8665 |
| Ensembl ID | ENSG00000175390 |
| UniProt ID | O00303 |
| OMIM ID | 603914 |
| HGNC ID | 3275 |
| Aliases | eIF3f, eIF3-p47, MGC102073, MGC126726, MGC126728 |
Description
EIF3F encodes a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex, which is essential for the initiation of protein synthesis. The eIF3 complex binds to the 40S ribosomal subunit and promotes the formation of the 43S pre-initiation complex. EIF3F is involved in regulating translation and has been implicated in cellular growth, proliferation, and apoptosis. Alterations in EIF3F expression or function are associated with various cancers and developmental disorders.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal cancer | Altered EIF3F expression affects translation of oncogenic mRNAs; loss of function may promote tumorigenesis | COSMIC, NCBI Gene |
| Breast cancer | EIF3F downregulation correlates with poor prognosis; may modulate translation of cell cycle regulators | NCBI Gene, PubMed |
| Hepatocellular carcinoma | EIF3F overexpression linked to increased proliferation and metastasis | COSMIC, NCBI Gene |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 15.2 | Medium |
| Thyroid | 12.8 | Medium |
| Adrenal gland | 10.5 | Medium |
| Lung | 9.3 | Low |
| Liver | 8.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 14.5 | Embryonic kidney; high expression |
| HeLa | 12.0 | Cervical carcinoma; moderate expression |
| HepG2 | 10.2 | Hepatocellular carcinoma; moderate expression |
| MCF7 | 9.8 | Breast cancer; moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.497C>T (p.Thr166Ile) | Missense | <0.01% | Unknown functional impact; rare variant |
| c.832G>A (p.Glu278Lys) | Missense | <0.01% | Reported in COSMIC; potential loss of function |
| c.1045_1047del (p.Lys349del) | In-frame deletion | <0.01% | May affect protein stability |
Mutation functional classification
Loss of Function (LOF)
Missense mutations (e.g., p.Glu278Lys) may impair eIF3 complex assembly or translation initiation activity.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No evidence of dominant-negative effects.
View complete mutation data：
Gene Ontology (GO)
| • translation initiation factor activity (GO:0003743) | • RNA binding (GO:0003723) |
| • cytoplasmic translation (GO:0002181) | • eIF3 complex (GO:0005852) |
Pathways
• Eukaryotic Translation Initiation (Reactome: R-HSA-72613)
• Cap-dependent Translation Initiation (Reactome: R-HSA-72737)
• Formation of the 43S pre-initiation complex (Reactome: R-HSA-72649)
Protein Summary
EIF3F is a 47 kDa protein subunit of the eIF3 complex. It contains a PCI domain (Proteasome, COP9, Initiation factor 3) that mediates protein-protein interactions within the complex. EIF3F is essential for stabilizing the eIF3 complex and facilitating mRNA recruitment to the ribosome. Post-translational modifications include phosphorylation, which may regulate its activity.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| EIF3F Knockout HEK293 Cell Line | EDJ-KQ50813 | Human | 8665 | Details Get a Quote |
| EIF3F Knockout HeLa Cell Line | EDJ-KQ54975 | Human | 8665 | Details Get a Quote |
| EIF3F Knockout A-549 Cell Line | EDJ-KQ63456 | Human | 8665 | Details Get a Quote |
| EIF3F Knockout HCT 116 Cell Line | EDJ-KQ71924 | Human | 8665 | Details Get a Quote |
