EHF (ETS Homologous Factor)
ETS transcription factor involved in epithelial development and cancer
Gene Information Card
| Symbol | EHF |
|---|---|
| Full Name | ETS homologous factor |
| Gene Type | protein-coding |
| Chromosomal Location | 11p13 |
| NCBI Gene ID | 26298 ncbi.nlm.nih.gov/gene/26298 |
| Ensembl ID | ENSG00000135318 |
| UniProt ID | Q9NZC4 |
| OMIM ID | 605439 |
| HGNC ID | 3246 |
| Aliases | ESE-3, ESE3, ETS homologous factor |
Description
EHF (ETS homologous factor) is a member of the ETS family of transcription factors, characterized by a conserved ETS DNA-binding domain. It acts as a transcriptional repressor or activator, playing a critical role in epithelial cell differentiation, proliferation, and homeostasis. EHF is involved in the development of various epithelial tissues and has been implicated in several cancers, including prostate, breast, and lung cancer, where it can function as a tumor suppressor or oncogene depending on context.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Prostate cancer | EHF expression is frequently lost in prostate cancer; loss promotes epithelial-mesenchymal transition and metastasis. | PMID: 22902505, PMID: 25944712 |
| Breast cancer | EHF acts as a tumor suppressor; reduced expression correlates with poor prognosis and aggressive subtypes. | PMID: 24803677 |
| Lung cancer | EHF is downregulated in non-small cell lung cancer; its loss contributes to invasion and migration. | PMID: 26921331 |
| Colorectal cancer | EHF expression is altered; may influence Wnt/β-catenin signaling. | PMID: 28263393 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Prostate | 12.5 | Medium |
| Lung | 8.3 | Medium |
| Breast | 6.1 | Low |
| Colon | 5.4 | Low |
| Skin | 4.8 | Low |
| Salivary gland | 3.2 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| PC-3 (prostate) | 2.1 | Low expression |
| MCF-7 (breast) | 1.8 | Low expression |
| A549 (lung) | 3.5 | Low expression |
| HCT116 (colon) | 4.0 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| p.R169* | Nonsense | <1% | Loss of function; truncation of ETS domain |
| p.E87K | Missense | <1% | Unknown; may affect DNA binding |
| p.S124L | Missense | <1% | Unknown; located in PNT domain |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations that truncate the ETS domain or lead to nonsense-mediated decay result in loss of transcriptional activity.
Gain of Function (GOF)
No well-characterized gain-of-function mutations reported in EHF.
Dominant Negative (DN)
Truncated isoforms lacking the transactivation domain may interfere with wild-type EHF function, but evidence is limited.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Epithelial-to-mesenchymal transition (EMT)
• Wnt/β-catenin signaling
• Androgen receptor signaling
Protein Summary
EHF encodes a 300-amino acid protein containing an N-terminal Pointed (PNT) domain and a C-terminal ETS DNA-binding domain. It localizes to the nucleus and binds to ETS recognition sites (GGAA/T) in target gene promoters. EHF regulates genes involved in cell adhesion, migration, and differentiation. Its expression is predominantly restricted to epithelial tissues, and loss of EHF is associated with increased invasiveness and metastatic potential in multiple cancer types.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| EHF Knockout HEK293 Cell Line | EDJ-KQ8512 | Human | 26298 | Details Get a Quote |
| EHF Knockout A-549 Cell Line | EDJ-KQ34640 | Human | 26298 | Details Get a Quote |
| EHF Knockout HCT 116 Cell Line | EDJ-KQ34641 | Human | 26298 | Details Get a Quote |
| EHF Knockout HeLa Cell Line | EDJ-KQ55928 | Human | 26298 | Details Get a Quote |
