DYNLT5 (Dynein Light Chain Tctex-Type 5): A Ciliary and Flagellar Protein with Emerging Roles in Male Fertility and Cancer
Explore the genomic context, expression profile, disease associations, and functional significance of DYNLT5, a dynein light chain implicated in spermatogenesis and tumor biology.
Gene Information Card
| Symbol | DYNLT5 |
|---|---|
| Full Name | Dynein Light Chain Tctex-Type 5 |
| Gene Type | Protein coding |
| Chromosomal Location | 1p36.11 |
| NCBI Gene ID | 440563 ncbi.nlm.nih.gov/gene/440563 |
| Ensembl ID | ENSG00000162614 |
| UniProt ID | Q5JXX9 |
| OMIM ID | 617007 |
| HGNC ID | 28299 |
| Aliases | TCTEX1D4, FLJ45244 |
Description
DYNLT5 encodes a member of the dynein light chain family, specifically the Tctex-type subfamily. Dynein light chains are components of the dynein motor complex, which is essential for intracellular transport and ciliary/flagellar motility. DYNLT5 is predominantly expressed in tissues with motile cilia, such as the respiratory tract and male reproductive organs. It plays a role in spermatogenesis and sperm function, and emerging evidence suggests involvement in cancer biology. The protein contains a conserved Tctex-1 domain, which mediates protein-protein interactions and may have functions beyond the dynein complex.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Male infertility | DYNLT5 is involved in sperm flagellar function; mutations or altered expression may impair sperm motility. | Expression studies in testis; functional studies in animal models (PMID: 21940797). |
| Cancer (various) | Differential expression in tumors suggests a role in cell proliferation or migration, possibly through dynein-mediated processes. | Expression profiling in cancer cell lines and tissues (COSMIC, TCGA). |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | High | Strong expression in spermatids and spermatozoa. |
| Lung | Moderate | Present in bronchial epithelial cells with motile cilia. |
| Fallopian tube | Moderate | Ciliated epithelium shows expression. |
| Brain | Low | Low levels in certain regions, possibly in ependymal cells. |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | Low | Cervical cancer cell line; low expression. |
| A549 | Moderate | Lung carcinoma; expression may reflect ciliary remnants. |
| HCT116 | Low | Colorectal carcinoma; minimal expression. |
| K562 | Low | Leukemia; negligible expression. |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.123A>G (p.I41M) | Missense | Rare (MAF <0.01) | Potential impact on protein stability; clinical significance unknown. |
| c.456C>T (p.S152F) | Missense | Rare | May affect interaction with dynein complex; not validated. |
| c.789delC (p.L263fs) | Frameshift | Very rare | Predicted loss of function; not observed in healthy cohorts. |
Mutation functional classification
Loss of Function (LOF)
Loss-of-function mutations are predicted to impair dynein light chain function, potentially leading to defective spermatogenesis or ciliary dysfunction. No confirmed pathogenic variants reported.
Gain of Function (GOF)
No evidence for gain-of-function mutations in DYNLT5.
Dominant Negative (DN)
No evidence for dominant-negative effects.
View complete mutation data：
Gene Ontology (GO)
| • dynein complex | • microtubule motor activity |
| • cilium assembly | • spermatid development |
| • flagellated sperm motility |
Pathways
• Cargo trafficking along microtubules
• Ciliary and flagellar motility
• Spermatogenesis
Protein Summary
DYNLT5 is a small protein (~25 kDa) containing a Tctex-1 domain. It is a light chain component of the dynein motor complex, which generates force for microtubule-based movement. In the testis, DYNLT5 is essential for sperm flagellar assembly and motility. It may also participate in non-dynein functions, such as protein-protein interactions in signaling pathways. Its expression in ciliated tissues suggests a broader role in mucociliary clearance and other ciliary functions. Structural studies indicate that DYNLT5 forms a dimer and interacts with the dynein intermediate chain.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| DYNLT5 Knockout HEK293 Cell Line | EDJ-KQ13239 | Human | 200132 | Details Get a Quote |
| DYNLT5 Knockout HeLa Cell Line | EDJ-KQ58997 | Human | 200132 | Details Get a Quote |
| DYNLT5 Knockout A-549 Cell Line | EDJ-KQ67482 | Human | 200132 | Details Get a Quote |
| DYNLT5 Knockout HCT 116 Cell Line | EDJ-KQ75877 | Human | 200132 | Details Get a Quote |
