DUSP16 (Dual Specificity Phosphatase 16)
A key regulator of MAPK signaling and stress response
Gene Information Card
| Symbol | DUSP16 |
|---|---|
| Full Name | Dual Specificity Phosphatase 16 |
| Gene Type | Protein coding |
| Chromosomal Location | 12p13.31 |
| NCBI Gene ID | 80824 ncbi.nlm.nih.gov/gene/80824 |
| Ensembl ID | ENSG00000111266 |
| UniProt ID | Q9BY84 |
| OMIM ID | 608203 |
| HGNC ID | 30713 |
| Aliases | MKP-7, MKP7, MGC126561 |
Description
DUSP16 encodes a dual specificity phosphatase that dephosphorylates phosphotyrosine and phosphoserine/threonine residues within MAP kinases, particularly JNK and p38, thereby negatively regulating MAPK signaling pathways. It plays a critical role in cellular stress responses, proliferation, and apoptosis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various) | DUSP16 overexpression or mutation alters JNK/p38 signaling, promoting tumorigenesis or chemoresistance | COSMIC, NCBI |
| Neurodevelopmental disorders | Loss-of-function variants impair MAPK regulation, linked to intellectual disability | ClinVar, OMIM |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 15.2 | Medium |
| Heart | 12.8 | Medium |
| Liver | 8.5 | Low |
| Kidney | 11.3 | Medium |
| Testis | 18.7 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 14.5 | High expression |
| HeLa | 10.2 | Moderate expression |
| K562 | 7.8 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1072C>T (p.Arg358Trp) | Missense | <0.01% | Loss of phosphatase activity |
| c.1456G>A (p.Glu486Lys) | Missense | <0.01% | Altered substrate specificity |
Mutation functional classification
Loss of Function (LOF)
Missense mutations in catalytic domain reduce JNK/p38 dephosphorylation, leading to sustained MAPK activation.
Gain of Function (GOF)
Not well documented; overexpression in some cancers may act as a gain-of-function.
Dominant Negative (DN)
Truncating or catalytic-dead mutants can interfere with wild-type DUSP16 function.
View complete mutation data：
Gene Ontology (GO)
| • MAP kinase phosphatase activity | • protein tyrosine phosphatase activity |
| • JNK cascade | • negative regulation of MAPK cascade |
| • cytoplasm | • nucleus |
Pathways
• MAPK signaling pathway
• JNK signaling
• p38 MAPK signaling
• Stress-activated protein kinase signaling
Protein Summary
DUSP16 (MKP-7) is a 665-amino acid dual specificity phosphatase that specifically inactivates JNK and p38 MAP kinases. It contains a N-terminal MAP kinase-binding domain and a C-terminal catalytic phosphatase domain. The protein shuttles between nucleus and cytoplasm, regulating stress-induced signaling.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| DUSP16 Knockout HEK293 Cell Line | EDJ-KQ156 | Human | 80824 | Details Get a Quote |
| DUSP16 Knockout A-549 Cell Line | EDJ-KQ19118 | Human | 80824 | Details Get a Quote |
| DUSP16 Knockout HCT 116 Cell Line | EDJ-KQ19119 | Human | 80824 | Details Get a Quote |
| DUSP16 Knockout HeLa Cell Line | EDJ-KQ19120 | Human | 80824 | Details Get a Quote |
