DEPTOR
DEP Domain Containing MTOR Interacting Protein
Gene Information Card
| Symbol | DEPTOR |
|---|---|
| Full Name | DEP domain containing MTOR interacting protein |
| Gene Type | protein-coding |
| Chromosomal Location | 8q24.12 |
| NCBI Gene ID | 64798 ncbi.nlm.nih.gov/gene/64798 |
| Ensembl ID | ENSG00000146648 |
| UniProt ID | Q8TB45 |
| OMIM ID | 612974 |
| HGNC ID | 22953 |
| Aliases | DEP.6, DEPDC6 |
Description
DEPTOR (DEP domain containing MTOR interacting protein) encodes a protein that binds to mTORC1 and mTORC2 complexes, inhibiting their kinase activity. It plays a critical role in regulating cell growth, proliferation, and survival. DEPTOR expression is frequently dysregulated in various cancers and is involved in the PI3K/AKT/mTOR signaling pathway.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Multiple Myeloma | DEPTOR overexpression activates mTOR signaling and promotes tumor growth | PMID: 21516109 |
| Prostate Cancer | DEPTOR loss leads to mTOR pathway hyperactivation and resistance to therapy | PMID: 25624456 |
| Breast Cancer | DEPTOR downregulation correlates with poor prognosis and increased AKT signaling | PMID: 23558955 |
| Lung Cancer | DEPTOR expression is reduced in tumors, contributing to mTORC1 activation | PMID: 24043774 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.3 | Medium |
| Adipose tissue | 8.5 | Low |
| Brain | 6.2 | Low |
| Liver | 4.1 | Low |
| Kidney | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 15.2 | High expression |
| HeLa | 10.5 | Moderate expression |
| MCF7 | 7.8 | Low expression |
| A549 | 6.3 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | missense | <0.01% | Loss of start codon, likely loss of function |
| c.100C>T (p.Arg34Trp) | missense | <0.01% | Unknown functional effect |
| c.200_201del (p.Glu67fs) | frameshift | <0.01% | Predicted loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations that truncate the protein or disrupt the DEP domain are predicted to impair mTOR inhibition.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported in DEPTOR.
Dominant Negative (DN)
No evidence of dominant-negative mutations in DEPTOR.
View complete mutation data：
Gene Ontology (GO)
Pathways
• PI3K-Akt signaling pathway (KEGG: hsa04151)
• mTOR signaling pathway (KEGG: hsa04150)
• Regulation of autophagy (Reactome: R-HSA-163285)
Protein Summary
DEPTOR is a 409-amino acid protein containing an N-terminal DEP domain and a C-terminal PDZ domain. It directly binds to mTOR and inhibits both mTORC1 and mTORC2 complexes. DEPTOR is regulated by ubiquitin-mediated degradation and is involved in feedback loops with AKT. Its expression is tightly controlled and dysregulation contributes to cancer progression.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| DEPTOR Knockout HEK293 Cell Line | EDJ-KQ1169 | Human | 64798 | Details Get a Quote |
| DEPTOR Knockout A-549 Cell Line | EDJ-KQ20427 | Human | 64798 | Details Get a Quote |
| DEPTOR Knockout HCT 116 Cell Line | EDJ-KQ20428 | Human | 64798 | Details Get a Quote |
| DEPTOR Knockout HeLa Cell Line | EDJ-KQ20429 | Human | 64798 | Details Get a Quote |
