DCAF15 Gene
DDB1 and CUL4 Associated Factor 15
Gene Information Card
| Symbol | DCAF15 |
|---|---|
| Full Name | DDB1 and CUL4 Associated Factor 15 |
| Gene Type | protein-coding |
| Chromosomal Location | 19p13.2 |
| NCBI Gene ID | 90379 ncbi.nlm.nih.gov/gene/90379 |
| Ensembl ID | ENSG00000167658 |
| UniProt ID | Q66K64 |
| OMIM ID | 617738 |
| HGNC ID | 26767 |
| Aliases | FLJ11273, MGC13170 |
Description
DCAF15 (DDB1 and CUL4 Associated Factor 15) encodes a substrate receptor for the CUL4-DDB1 E3 ubiquitin ligase complex. It is involved in ubiquitination and proteasomal degradation of target proteins, playing a role in cell cycle regulation and DNA damage response. DCAF15 is also a target of the anticancer drug indisulam, which promotes degradation of splicing factors RBM39 and RBM23.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Acute Myeloid Leukemia | Indisulam-induced degradation of RBM39 via DCAF15 leads to splicing inhibition and cell death | PMID: 28263314 |
| Colorectal Cancer | DCAF15 overexpression associated with poor prognosis; potential therapeutic target | PMID: 31695179 |
| Breast Cancer | DCAF15 expression correlates with tumor grade and survival | PMID: 32620804 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Bone Marrow | 8.3 | Low |
| Lymph Node | 6.1 | Low |
| Brain | 4.2 | Low |
| Liver | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 15.2 | Embryonic kidney |
| K562 | 10.1 | Leukemia |
| HCT116 | 9.5 | Colorectal carcinoma |
| MCF7 | 7.8 | Breast cancer |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Thr34Met) | Missense | 0.02% (gnomAD) | Unknown |
| c.457G>A (p.Gly153Ser) | Missense | 0.01% (gnomAD) | Unknown |
| c.784C>T (p.Arg262Trp) | Missense | 0.005% (gnomAD) | Unknown |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in COSMIC or ClinVar.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No confirmed dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • ubiquitin-protein transferase activity | • protein ubiquitination |
| • DDB1-CUL4 E3 ubiquitin ligase complex | • cell cycle |
| • DNA repair |
Pathways
• CUL4-DDB1 E3 ubiquitin ligase pathway
• Ubiquitin-mediated proteolysis
Protein Summary
DCAF15 is a 546-amino acid protein containing WD40 repeats that mediate substrate recognition. It forms a complex with DDB1 and CUL4 to ubiquitinate target proteins, including splicing factors RBM39 and RBM23. The protein is expressed in multiple tissues and is implicated in cancer cell survival.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| DCAF15 Knockout HEK293 Cell Line | EDJ-KQ10594 | Human | 90379 | Details Get a Quote |
| DCAF15 Knockout A-549 Cell Line | EDJ-KQ38071 | Human | 90379 | Details Get a Quote |
| DCAF15 Knockout HCT 116 Cell Line | EDJ-KQ38072 | Human | 90379 | Details Get a Quote |
| DCAF15 Knockout HeLa Cell Line | EDJ-KQ38073 | Human | 90379 | Details Get a Quote |
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