DCAF1
DDB1 and CUL4 Associated Factor 1
Gene Information Card
| Symbol | DCAF1 |
|---|---|
| Full Name | DDB1 and CUL4 Associated Factor 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 3p21.31 |
| NCBI Gene ID | 64759 ncbi.nlm.nih.gov/gene/64759 |
| Ensembl ID | ENSG00000163930 |
| UniProt ID | Q9Y4B6 |
| OMIM ID | 613200 |
| HGNC ID | 23411 |
| Aliases | VPRBP, RIP, DDB1- and CUL4-associated factor 1 |
Description
DCAF1 (DDB1 and CUL4 Associated Factor 1) encodes a substrate receptor for the CUL4-DDB1 E3 ubiquitin ligase complex. It targets proteins for ubiquitination and proteasomal degradation, playing roles in cell cycle regulation, DNA replication, and chromatin remodeling. DCAF1 is also exploited by HIV-1 Vpr to induce cell cycle arrest and promote viral replication.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various types) | DCAF1 overexpression or mutation alters ubiquitination of tumor suppressors (e.g., p53, MCM10), promoting genomic instability and proliferation. | PMID: 23541922, COSMIC |
| HIV-1 infection | HIV-1 Vpr binds DCAF1 to recruit the CRL4-DCAF1 complex, leading to degradation of host restriction factors and G2/M cell cycle arrest. | PMID: 17693256, 17981126 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 15.2 | Medium |
| Lymph node | 12.8 | Medium |
| Bone marrow | 11.5 | Medium |
| Brain | 8.3 | Low |
| Liver | 6.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 14.5 | Embryonic kidney |
| HeLa | 12.3 | Cervical carcinoma |
| K562 | 10.8 | Leukemia |
| HepG2 | 9.2 | Hepatocellular carcinoma |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T (p.Arg412Trp) | Missense | <0.1% (COSMIC) | Unknown; predicted to affect substrate binding |
| c.567_569del (p.Lys189del) | In-frame deletion | <0.1% (COSMIC) | Unknown; may alter protein stability |
Mutation functional classification
Loss of Function (LOF)
Not well characterized; rare truncating mutations may impair CRL4 complex assembly.
Gain of Function (GOF)
Not reported.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
| • ubiquitin protein ligase activity | • protein binding |
| • chromatin binding | • cell cycle |
| • DNA replication | • viral process |
Pathways
• CRL4-DDB1 E3 ubiquitin ligase complex
• HIV-1 Vpr-mediated cell cycle arrest
Protein Summary
DCAF1 is a 1507-amino acid protein containing WD40 repeats that mediate substrate recognition. It serves as a substrate receptor for the CUL4-DDB1 ubiquitin ligase complex, targeting proteins such as MCM10 and p53 for degradation. DCAF1 is essential for DNA replication and cell cycle progression, and its interaction with HIV-1 Vpr is critical for viral pathogenesis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| DCAF12 Knockout HEK293 Cell Line | EDJ-KQ2888 | Human | 25853 | Details Get a Quote |
| DCAF11 Knockout HEK293 Cell Line | EDJ-KQ3877 | Human | 80344 | Details Get a Quote |
| DCAF1 Knockout HEK293 Cell Line | EDJ-KQ3922 | Human | 9730 | Details Get a Quote |
| DCAF13 Knockout HEK293 Cell Line | EDJ-KQ8278 | Human | 25879 | Details Get a Quote |
| DCAF17 Knockout HEK293 Cell Line | EDJ-KQ9441 | Human | 80067 | Details Get a Quote |
| DCAF12L1 Knockout HEK293 Cell Line | EDJ-KQ9523 | Human | 139170 | Details Get a Quote |
| DCAF15 Knockout HEK293 Cell Line | EDJ-KQ10594 | Human | 90379 | Details Get a Quote |
| DCAF10 Knockout HEK293 Cell Line | EDJ-KQ13100 | Human | 79269 | Details Get a Quote |
| DCAF12L2 Knockout HEK293 Cell Line | EDJ-KQ13101 | Human | 340578 | Details Get a Quote |
| DCAF16 Knockout HEK293 Cell Line | EDC90528 | Human | 54876 | Details Get a Quote |
| DCAF11 Knockout A-549 Cell Line | EDJ-KQ26085 | Human | 80344 | Details Get a Quote |
| DCAF11 Knockout HeLa Cell Line | EDJ-KQ26087 | Human | 80344 | Details Get a Quote |
| DCAF1 Knockout A-549 Cell Line | EDJ-KQ26154 | Human | 9730 | Details Get a Quote |
| DCAF1 Knockout HCT 116 Cell Line | EDJ-KQ26155 | Human | 9730 | Details Get a Quote |
| DCAF1 Knockout HeLa Cell Line | EDJ-KQ26156 | Human | 9730 | Details Get a Quote |
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