DAPK3
Death-Associated Protein Kinase 3: A Key Regulator of Apoptosis and Smooth Muscle Contraction
Gene Information Card
| Symbol | DAPK3 |
|---|---|
| Full Name | death-associated protein kinase 3 |
| Gene Type | protein-coding |
| Chromosomal Location | 19p13.3 |
| NCBI Gene ID | 1613 ncbi.nlm.nih.gov/gene/1613 |
| Ensembl ID | ENSG00000167657 |
| UniProt ID | O43293 |
| OMIM ID | 603289 |
| HGNC ID | 2676 |
| Aliases | ZIPK, ZIP kinase, DAPK3, DAP-3, DAP-like kinase |
Description
DAPK3 (death-associated protein kinase 3) encodes a serine/threonine protein kinase that belongs to the DAPK family. The protein is involved in multiple cellular processes including apoptosis, autophagy, and smooth muscle contraction. It acts as a tumor suppressor by promoting cell death and is also a key regulator of actomyosin contractility through phosphorylation of myosin light chain. DAPK3 is widely expressed and its dysregulation is implicated in various cancers and cardiovascular disorders.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Bladder cancer | Loss of DAPK3 expression via promoter methylation reduces apoptosis, promoting tumorigenesis | ClinVar, COSMIC |
| Colorectal cancer | Somatic mutations and decreased expression contribute to tumor progression | COSMIC, NCBI |
| Prostate cancer | Epigenetic silencing of DAPK3 correlates with aggressive disease | NCBI, OMIM |
| Cardiovascular disease | Altered DAPK3 activity affects smooth muscle contraction and vascular tone | UniProt, OMIM |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 10.2 | Medium |
| Heart | 8.5 | Medium |
| Liver | 6.1 | Low |
| Lung | 7.8 | Medium |
| Kidney | 9.3 | Medium |
| Skeletal muscle | 12.4 | High |
| Smooth muscle | 15.0 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 11.5 | Cervical cancer cell line |
| A549 | 9.8 | Lung cancer cell line |
| MCF7 | 7.2 | Breast cancer cell line |
| HCT116 | 8.9 | Colorectal cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | missense | Rare | Loss of start codon, likely loss of function |
| c.374C>T (p.Thr125Met) | missense | 0.01% | Reduced kinase activity |
| c.682G>A (p.Glu228Lys) | missense | 0.005% | Altered substrate binding |
Mutation functional classification
Loss of Function (LOF)
Mutations that abolish kinase activity or protein expression, such as start codon loss or truncating variants, are associated with reduced apoptosis and increased cancer risk.
Gain of Function (GOF)
No well-characterized gain-of-function mutations reported in DAPK3.
Dominant Negative (DN)
Some missense mutations may interfere with dimerization or substrate binding, potentially acting in a dominant-negative manner.
View complete mutation data：
Gene Ontology (GO)
| • protein serine/threonine kinase activity | • apoptotic process |
| • smooth muscle contraction | • protein phosphorylation |
| • regulation of cell death | • myosin light chain binding |
Pathways
• Apoptosis
• Smooth muscle contraction
• p53 signaling pathway
• TNF signaling pathway
Protein Summary
DAPK3 is a 454-amino acid serine/threonine kinase with an N-terminal kinase domain, a leucine zipper domain, and a C-terminal calmodulin-binding region. It phosphorylates myosin light chain and other substrates to regulate actomyosin contractility. The protein is activated by dimerization and autophosphorylation, and it promotes apoptosis through interaction with DAPK1 and other death-associated proteins. DAPK3 is widely expressed in human tissues, with highest levels in smooth and skeletal muscle.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| DAPK3 Knockout HEK293 Cell Line | EDJ-KQ4421 | Human | 1613 | Details Get a Quote |
| DAPK3 Knockout A-549 Cell Line | EDJ-KQ26949 | Human | 1613 | Details Get a Quote |
| DAPK3 Knockout HCT 116 Cell Line | EDJ-KQ26950 | Human | 1613 | Details Get a Quote |
| DAPK3 Knockout HeLa Cell Line | EDJ-KQ25682 | Human | 1613 | Details Get a Quote |
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