CXCL8 (Interleukin-8) Gene
Chemokine (C-X-C motif) ligand 8: Key mediator of inflammation and neutrophil chemotaxis
Gene Information Card
| Symbol | CXCL8 |
|---|---|
| Full Name | C-X-C motif chemokine ligand 8 |
| Gene Type | protein-coding |
| Chromosomal Location | 4q13.3 |
| NCBI Gene ID | 3576 ncbi.nlm.nih.gov/gene/3576 |
| Ensembl ID | ENSG00000169429 |
| UniProt ID | P10145 |
| OMIM ID | 146930 |
| HGNC ID | 6025 |
| Aliases | IL8, NAF, GCP1, LECT, LUCT, NAP1, SCYB8, TSG1, b-ENAP, CXCL8 |
Description
The CXCL8 gene encodes interleukin-8 (IL-8), a member of the CXC chemokine family. IL-8 is a potent chemoattractant for neutrophils, basophils, and T-cells, and plays a critical role in the inflammatory response, angiogenesis, and tumor progression. It is produced by multiple cell types including macrophages, epithelial cells, and endothelial cells in response to inflammatory stimuli such as TNF-alpha, IL-1, and bacterial products.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Chronic obstructive pulmonary disease (COPD) | Elevated CXCL8 expression recruits neutrophils to airways, contributing to tissue damage and inflammation. | NCBI Gene, OMIM |
| Rheumatoid arthritis | Overexpression of CXCL8 in synovial fluid promotes neutrophil infiltration and joint destruction. | NCBI Gene, OMIM |
| Colorectal cancer | CXCL8 acts as a growth factor and angiogenic factor, promoting tumor progression and metastasis. | COSMIC, NCBI Gene |
| Bronchial asthma | Increased CXCL8 levels correlate with airway neutrophilia and disease severity. | NCBI Gene, OMIM |
| Sepsis | Systemic elevation of CXCL8 contributes to neutrophil-mediated organ damage. | NCBI Gene |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Lung | 12.5 | High |
| Bone marrow | 8.3 | Medium |
| Spleen | 6.1 | Medium |
| Whole blood | 4.8 | Medium |
| Lymph node | 3.2 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| THP-1 (monocytic leukemia) | 15.2 | High expression after LPS stimulation |
| A549 (lung carcinoma) | 10.7 | Constitutive and inducible |
| HUVEC (endothelial) | 7.9 | Induced by TNF-alpha |
| HeLa (cervical carcinoma) | 5.4 | Moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.251A>G (p.Lys84Arg) | Missense | <0.1% | Reduced chemotactic activity in vitro |
| c.296C>T (p.Thr99Met) | Missense | <0.1% | Altered receptor binding affinity |
| c.373G>A (p.Gly125Ser) | Missense | <0.1% | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
Rare missense variants (e.g., p.Lys84Arg) reduce neutrophil chemotaxis and receptor activation.
Gain of Function (GOF)
No well-characterized gain-of-function mutations reported in CXCL8.
Dominant Negative (DN)
Not described for CXCL8.
View complete mutation data：
Gene Ontology (GO)
| • chemokine activity | • CXCR chemokine receptor binding |
| • inflammatory response | • neutrophil chemotaxis |
| • angiogenesis | • positive regulation of cell proliferation |
| • cellular response to interleukin-1 | • extracellular space |
Pathways
• IL-8 signaling pathway (Reactome: R-HSA-6783783)
• Chemokine signaling pathway (KEGG: hsa04062)
• TNF signaling pathway (KEGG: hsa04668)
• Toll-like receptor signaling pathway (KEGG: hsa04620)
• NF-kappa B signaling pathway (KEGG: hsa04064)
Protein Summary
Interleukin-8 (IL-8) is a 99-amino-acid protein (UniProt P10145) that is secreted as a homodimer. It binds to CXCR1 and CXCR2 receptors on neutrophils, triggering chemotaxis, degranulation, and respiratory burst. IL-8 is also implicated in tumor angiogenesis and metastasis. The protein contains a characteristic CXC motif (Cys-X-Cys) essential for receptor interaction.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CXCL8 Knockout HEK293 Cell Line | EDJ-KQ559 | Human | 3576 | Details Get a Quote |
| CXCL8 Knockout HCT 116 Cell Line | EDJ-KQ18029 | Human | 3576 | Details Get a Quote |
| CXCL8 Knockout A-549 Cell Line | EDJ-KQ18936 | Human | 3576 | Details Get a Quote |
| CXCL8 Knockout HeLa Cell Line | EDJ-KQ18938 | Human | 3576 | Details Get a Quote |
Displaying Records 1 To 4 Of 4 Records
