CPA4 Gene - Carboxypeptidase A4
A metallocarboxypeptidase involved in protein digestion, hormone processing, and cancer progression.
Gene Information Card
| Symbol | CPA4 |
|---|---|
| Full Name | Carboxypeptidase A4 |
| Gene Type | Protein coding |
| Chromosomal Location | 7q32.2 |
| NCBI Gene ID | 51200 ncbi.nlm.nih.gov/gene/51200 |
| Ensembl ID | ENSG00000128564 |
| UniProt ID | Q9UI42 |
| OMIM ID | 607635 |
| HGNC ID | 2296 |
| Aliases | CPA3, GPCR, carboxypeptidase A3 |
Description
CPA4 (Carboxypeptidase A4) encodes a member of the metallocarboxypeptidase family that cleaves C-terminal aromatic or branched-chain amino acids from proteins. It is secreted as a zymogen and activated by proteolytic cleavage. CPA4 is involved in digestive peptide processing, hormone maturation, and has been implicated in tumor progression, particularly in pancreatic and prostate cancers.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Pancreatic cancer | Overexpression promotes cell invasion and metastasis; may act via ECM remodeling | PMID: 21912567 |
| Prostate cancer | Upregulated in androgen-independent prostate cancer; associated with poor prognosis | PMID: 16397024 |
| Colorectal cancer | Increased expression correlates with lymph node metastasis and advanced stage | PMID: 25642768 |
| Hepatocellular carcinoma | CPA4 overexpression linked to tumor size and vascular invasion | PMID: 28289163 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Pancreas | 45.2 | High |
| Prostate | 12.8 | Medium |
| Liver | 6.5 | Low |
| Colon | 4.1 | Low |
| Kidney | 2.3 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| PANC-1 (pancreatic cancer) | 38.5 | High expression |
| LNCaP (prostate cancer) | 22.1 | Androgen-responsive |
| HepG2 (liver cancer) | 9.8 | Moderate expression |
| HT-29 (colorectal cancer) | 7.3 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.433G>A (p.Gly145Arg) | Missense | <0.01% | Unknown; predicted benign |
| c.1012C>T (p.Arg338Cys) | Missense | <0.01% | Unknown; predicted possibly damaging |
| c.1246_1248del (p.Lys416del) | In-frame deletion | <0.01% | Unknown; may affect protein stability |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in ClinVar or COSMIC.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No evidence of dominant-negative effects.
View complete mutation data：
Gene Ontology (GO)
| • metallocarboxypeptidase activity (GO:0004181) | • proteolysis (GO:0006508) |
| • extracellular region (GO:0005576) | • extracellular space (GO:0005615) |
| • zinc ion binding (GO:0008270) |
Pathways
• Peptide hormone processing (Reactome: R-HSA-422085)
• Collagen degradation (Reactome: R-HSA-1474228)
• Extracellular matrix organization (Reactome: R-HSA-1474244)
Protein Summary
CPA4 is a 421-amino acid secreted metallocarboxypeptidase that requires zinc for catalytic activity. It is synthesized as a preproprotein and activated by removal of the signal peptide and propeptide. The mature enzyme specifically cleaves C-terminal aromatic and branched-chain amino acids. CPA4 is highly expressed in the pancreas and prostate, and its overexpression in several cancers suggests a role in tumor invasion and metastasis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CPA4 Knockout HEK293 Cell Line | EDJ-KQ3017 | Human | 51200 | Details Get a Quote |
| CPA4 Knockout A-549 Cell Line | EDJ-KQ24230 | Human | 51200 | Details Get a Quote |
| CPA4 Knockout HCT 116 Cell Line | EDJ-KQ24231 | Human | 51200 | Details Get a Quote |
| CPA4 Knockout HeLa Cell Line | EDJ-KQ24232 | Human | 51200 | Details Get a Quote |
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