CLK3: CDC Like Kinase 3
A dual-specificity kinase regulating alternative splicing and gene expression
Gene Information Card
| Symbol | CLK3 |
|---|---|
| Full Name | CDC like kinase 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 15q24.1 |
| NCBI Gene ID | 1198 ncbi.nlm.nih.gov/gene/1198 |
| Ensembl ID | ENSG00000141384 |
| UniProt ID | P49761 |
| OMIM ID | 602987 |
| HGNC ID | 2068 |
| Aliases | PHCLK3, CLK, hCLK3 |
Description
CLK3 (CDC like kinase 3) is a member of the CDC2-like kinase family. It encodes a dual-specificity kinase that phosphorylates serine/arginine-rich (SR) proteins, thereby regulating alternative splicing. CLK3 is involved in cell cycle control, RNA processing, and gene expression. The protein contains a nuclear localization signal and is predominantly nuclear. Alternative splicing of the CLK3 gene itself produces multiple transcript variants.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Overexpression of CLK3 alters splicing of oncogenes and tumor suppressors | COSMIC; PMID: 25691885 |
| Colorectal cancer | CLK3 upregulation associated with poor prognosis and splicing dysregulation | COSMIC; PMID: 30948276 |
| Hepatocellular carcinoma | CLK3 promotes cell proliferation via SR protein phosphorylation | COSMIC; PMID: 31570831 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 28.1 | High |
| Lymph node | 18.5 | Medium |
| Brain | 15.2 | Medium |
| Liver | 10.3 | Low |
| Heart | 8.7 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 22.4 | Embryonic kidney, high expression |
| HeLa | 19.8 | Cervical carcinoma, moderate |
| MCF7 | 16.3 | Breast cancer, moderate |
| HepG2 | 12.1 | Hepatocellular carcinoma, low |
| K562 | 9.5 | Leukemia, low |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.112G>A (p.Glu38Lys) | Missense | 0.02% | Unknown functional impact |
| c.487C>T (p.Arg163Trp) | Missense | 0.01% | Potential loss of kinase activity |
| c.784_785insA (p.Thr262Asnfs*5) | Frameshift | <0.01% | Loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations that truncate the kinase domain are predicted to cause loss of function.
Gain of Function (GOF)
No gain-of-function mutations have been reported in CLK3.
Dominant Negative (DN)
No dominant-negative mutations have been characterized for CLK3.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Alternative splicing regulation (Reactome: R-HSA-72172)
• mRNA Splicing - Major Pathway (Reactome: R-HSA-72163)
• SRP-dependent cotranslational protein targeting to membrane (Reactome: R-HSA-1799339)
Protein Summary
CLK3 is a 638-amino acid dual-specificity kinase that phosphorylates serine, threonine, and tyrosine residues. It contains an N-terminal kinase domain and a C-terminal RS domain rich in arginine/serine dipeptides. The protein localizes to nuclear speckles and interacts with SR splicing factors. CLK3 autophosphorylates and regulates its own stability. It plays a role in cell cycle progression and apoptosis.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CLK3 Knockout HEK293 Cell Line | EDJ-KQ2565 | Human | 1198 | Details Get a Quote |
| CLK3 Knockout A-549 Cell Line | EDJ-KQ23230 | Human | 1198 | Details Get a Quote |
| CLK3 Knockout HCT 116 Cell Line | EDJ-KQ23231 | Human | 1198 | Details Get a Quote |
| CLK3 Knockout HeLa Cell Line | EDJ-KQ23232 | Human | 1198 | Details Get a Quote |
