CKAP2: Cytoskeleton-Associated Protein 2
A microtubule-stabilizing protein involved in cell cycle progression and implicated in cancer
Gene Information Card
| Symbol | CKAP2 |
|---|---|
| Full Name | Cytoskeleton Associated Protein 2 |
| Gene Type | Protein coding |
| Chromosomal Location | 13q14.3 |
| NCBI Gene ID | 26586 ncbi.nlm.nih.gov/gene/26586 |
| Ensembl ID | ENSG00000136108 |
| UniProt ID | Q8WWK9 |
| OMIM ID | 611569 |
| HGNC ID | 1990 |
| Aliases | TMAP, LB-1, FLJ10706 |
Description
CKAP2 (Cytoskeleton Associated Protein 2) encodes a microtubule-associated protein that localizes to the mitotic spindle and centrosomes. It stabilizes microtubules and is required for proper cell cycle progression, particularly during mitosis. Expression is cell cycle-dependent, peaking in G2/M phase. CKAP2 is overexpressed in various cancers and may contribute to tumorigenesis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Glioma | Overexpression promotes cell proliferation and invasion | PMID: 25944712 |
| Lung cancer | Upregulation associated with poor prognosis | PMID: 23573269 |
| Breast cancer | High expression correlates with aggressive subtypes | PMID: 29228537 |
| Colorectal cancer | CKAP2 knockdown reduces tumor growth in vitro | PMID: 27323851 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Bone marrow | 8.2 | Low |
| Lymph node | 6.1 | Low |
| Brain | 4.3 | Low |
| Lung | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.2 | Cervical cancer cell line |
| A549 | 10.1 | Lung cancer cell line |
| MCF7 | 8.7 | Breast cancer cell line |
| U87MG | 12.3 | Glioblastoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1045C>T (p.Arg349Trp) | Missense | <0.01% | Unknown functional impact |
| c.1234G>A (p.Glu412Lys) | Missense | <0.01% | Reported in COSMIC |
| c.789_790insA | Frameshift | <0.01% | Predicted loss of function |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations are predicted to cause loss of function, potentially impairing mitotic progression.
Gain of Function (GOF)
Overexpression in tumors suggests possible gain-of-function in cancer contexts, but specific activating mutations are not well characterized.
Dominant Negative (DN)
No dominant-negative mutations have been reported for CKAP2.
View complete mutation data：
Gene Ontology (GO)
| • microtubule binding | • microtubule cytoskeleton organization |
| • mitotic spindle organization | • cell cycle |
| • mitosis | • centrosome |
| • spindle pole |
Pathways
• Cell Cycle
• Mitotic
• Microtubule-dependent transport
Protein Summary
CKAP2 is a 683-amino acid protein (UniProt Q8WWK9) that binds and stabilizes microtubules. It contains a coiled-coil domain and localizes to the mitotic spindle and centrosomes during mitosis. The protein is phosphorylated during the cell cycle and is degraded after mitosis. CKAP2 interacts with tubulin and other microtubule-associated proteins to regulate spindle dynamics.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CKAP2 Knockout HEK293 Cell Line | EDJ-KQ8563 | Human | 26586 | Details Get a Quote |
| CKAP2L Knockout HEK293 Cell Line | EDJ-KQ11301 | Human | 150468 | Details Get a Quote |
| CKAP2 Knockout A-549 Cell Line | EDJ-KQ34721 | Human | 26586 | Details Get a Quote |
| CKAP2 Knockout HCT 116 Cell Line | EDJ-KQ34722 | Human | 26586 | Details Get a Quote |
| CKAP2 Knockout HeLa Cell Line | EDJ-KQ34723 | Human | 26586 | Details Get a Quote |
| CKAP2L Knockout A-549 Cell Line | EDJ-KQ39422 | Human | 150468 | Details Get a Quote |
| CKAP2L Knockout HCT 116 Cell Line | EDJ-KQ39423 | Human | 150468 | Details Get a Quote |
| CKAP2L Knockout HeLa Cell Line | EDJ-KQ39424 | Human | 150468 | Details Get a Quote |
