CHST4: Carbohydrate Sulfotransferase 4
Gene encoding an enzyme involved in sulfation of carbohydrates, with roles in immune cell trafficking and cancer.
Gene Information Card
| Symbol | CHST4 |
|---|---|
| Full Name | Carbohydrate Sulfotransferase 4 |
| Gene Type | protein-coding |
| Chromosomal Location | 16q22.3 |
| NCBI Gene ID | 10164 ncbi.nlm.nih.gov/gene/10164 |
| Ensembl ID | ENSG00000140987 |
| UniProt ID | Q8NCG5 |
| OMIM ID | 603797 |
| HGNC ID | 1972 |
| Aliases | GST-3, HEC-GlcNAc6ST, L-selectin ligand sulfotransferase |
Description
CHST4 encodes a Golgi membrane sulfotransferase that catalyzes the transfer of sulfate to N-acetylglucosamine residues on glycoproteins. This sulfation is critical for the biosynthesis of L-selectin ligands on high endothelial venules, enabling lymphocyte homing to lymph nodes. The enzyme is also implicated in tumor progression and metastasis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal cancer | Altered CHST4 expression may affect L-selectin-mediated immune surveillance and tumor cell adhesion. | PMID: 25691885 |
| Breast cancer | CHST4 upregulation correlates with metastasis and poor prognosis. | PMID: 29348692 |
| Inflammatory bowel disease | Dysregulated sulfation of mucosal glycoproteins may alter immune cell recruitment. | PMID: 22033931 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Lymph node | 12.5 | Medium |
| Spleen | 8.3 | Low |
| Colon | 6.1 | Low |
| Breast | 4.2 | Low |
| Lung | 3.0 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 15.2 | High expression in transfected cells |
| HCT 116 | 7.8 | Moderate expression in colorectal cancer line |
| MCF7 | 5.1 | Low expression in breast cancer line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Thr34Met) | Missense | <0.01% | Unknown functional effect |
| c.457G>A (p.Gly153Ser) | Missense | <0.01% | Potential loss of sulfotransferase activity |
Mutation functional classification
Loss of Function (LOF)
Missense variants in the catalytic domain may reduce sulfotransferase activity, impairing L-selectin ligand synthesis.
Gain of Function (GOF)
Not reported.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• L-selectin ligand biosynthesis (Reactome: R-HSA-2024096)
• Sulfation of glycans (KEGG: map00533)
Protein Summary
CHST4 is a type II transmembrane protein localized to the Golgi apparatus. It transfers sulfate from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to the 6-position of N-acetylglucosamine on glycoproteins. This modification is essential for the generation of functional L-selectin ligands on high endothelial venules, facilitating lymphocyte homing. The enzyme is also expressed in certain cancers, where it may modulate tumor-immune interactions.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CHST4 Knockout HEK293 Cell Line | EDJ-KQ6931 | Human | 10164 | Details Get a Quote |
| CHST4 Knockout HCT 116 Cell Line | EDJ-KQ31582 | Human | 10164 | Details Get a Quote |
| CHST4 Knockout HeLa Cell Line | EDJ-KQ55332 | Human | 10164 | Details Get a Quote |
| CHST4 Knockout A-549 Cell Line | EDJ-KQ63813 | Human | 10164 | Details Get a Quote |
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