CHST2: Carbohydrate Sulfotransferase 2
Gene encoding an enzyme involved in sulfation of glycosaminoglycans and glycoproteins, with roles in cell signaling and cancer progression.
Gene Information Card
| Symbol | CHST2 |
|---|---|
| Full Name | Carbohydrate Sulfotransferase 2 |
| Gene Type | Protein coding |
| Chromosomal Location | 3q24 |
| NCBI Gene ID | 9435 ncbi.nlm.nih.gov/gene/9435 |
| Ensembl ID | ENSG00000163631 |
| UniProt ID | Q9Y4C5 |
| OMIM ID | 603798 |
| HGNC ID | 1970 |
| Aliases | GST-2, GnTST, GST2, N-acetylglucosamine-6-O-sulfotransferase |
Description
CHST2 encodes a Golgi membrane-bound sulfotransferase that catalyzes the transfer of sulfate to position 6 of N-acetylglucosamine residues in keratan sulfate and other glycoconjugates. This enzyme is involved in the biosynthesis of sulfated glycoproteins and glycosaminoglycans, influencing cell adhesion, migration, and signaling. CHST2 expression is associated with various cancers and developmental processes.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Altered sulfation of cell surface glycoproteins may promote tumor progression and metastasis. | PMID: 25691885 |
| Colorectal cancer | CHST2 overexpression correlates with poor prognosis and increased cell proliferation. | PMID: 29367642 |
| Lung cancer | CHST2-mediated sulfation affects growth factor signaling pathways. | PMID: 31073040 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Thyroid | 12.5 | Medium |
| Adrenal gland | 8.3 | Medium |
| Prostate | 6.7 | Medium |
| Lung | 5.1 | Low |
| Colon | 4.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 15.2 | High expression |
| HCT116 (colorectal cancer) | 12.8 | High expression |
| A549 (lung cancer) | 9.4 | Medium expression |
| HEK293 (embryonic kidney) | 6.1 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1045G>A (p.Gly349Arg) | Missense | <0.01% | Unknown functional impact |
| c.1237C>T (p.Arg413Trp) | Missense | <0.01% | Potential loss of sulfotransferase activity |
Mutation functional classification
Loss of Function (LOF)
Missense mutations in the catalytic domain may reduce sulfotransferase activity.
Gain of Function (GOF)
Not reported.
Dominant Negative (DN)
Not reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Keratan sulfate biosynthesis (Reactome: R-HSA-2022857)
• Sulfation of glycans (Reactome: R-HSA-2022854)
• Glycosaminoglycan metabolism (KEGG: hsa00532)
Protein Summary
CHST2 is a type II transmembrane protein localized to the Golgi apparatus. It contains a sulfotransferase domain that transfers sulfate from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to the 6-hydroxyl group of N-acetylglucosamine residues. The enzyme is critical for the synthesis of keratan sulfate and sulfated glycoproteins, modulating cell surface interactions and signaling. Structural studies indicate a conserved PAPS-binding motif essential for catalytic activity.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CHST2 Knockout HEK293 Cell Line | EDJ-KQ6586 | Human | 9435 | Details Get a Quote |
| CHST2 Knockout HCT 116 Cell Line | EDJ-KQ30808 | Human | 9435 | Details Get a Quote |
| CHST2 Knockout HeLa Cell Line | EDJ-KQ55164 | Human | 9435 | Details Get a Quote |
| CHST2 Knockout A-549 Cell Line | EDJ-KQ63644 | Human | 9435 | Details Get a Quote |
Displaying Records 1 To 4 Of 4 Records
