CDC25B: Cell Division Cycle 25B Phosphatase
A dual-specificity phosphatase regulating cell cycle progression and a target in cancer research.
Gene Information Card
| Symbol | CDC25B |
|---|---|
| Full Name | Cell Division Cycle 25B |
| Gene Type | protein-coding |
| Chromosomal Location | 20p13 |
| NCBI Gene ID | 994 ncbi.nlm.nih.gov/gene/994 |
| Ensembl ID | ENSG00000101224 |
| UniProt ID | P30305 |
| OMIM ID | 116949 |
| HGNC ID | 1727 |
| Aliases | CDC25B2, CDC25HU2, M-Phase Inducer Phosphatase 2 |
Description
CDC25B is a dual-specificity phosphatase that activates cyclin-dependent kinases (CDKs) by dephosphorylating inhibitory phosphotyrosine and phosphothreonine residues. It plays a critical role in the G2/M transition of the cell cycle and is involved in DNA damage checkpoint recovery. Overexpression and mutations are associated with various cancers.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast Cancer | Overexpression of CDC25B leads to unscheduled CDK1 activation, promoting cell cycle progression and tumor growth. | NCBI Gene, COSMIC |
| Colorectal Cancer | CDC25B overexpression correlates with poor prognosis and increased proliferation. | NCBI Gene, COSMIC |
| Non-Small Cell Lung Cancer | Elevated CDC25B expression is linked to aggressive tumor behavior and resistance to therapy. | NCBI Gene, COSMIC |
| Prostate Cancer | CDC25B upregulation contributes to androgen-independent growth. | NCBI Gene, COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Bone Marrow | 8.3 | Medium |
| Lymph Node | 6.1 | Low |
| Spleen | 5.4 | Low |
| Brain | 2.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.2 | Cervical cancer cell line; high expression |
| MCF7 | 10.8 | Breast cancer cell line; moderate expression |
| A549 | 9.5 | Lung cancer cell line; moderate expression |
| HCT116 | 7.3 | Colorectal cancer cell line; low-moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.473C>T (p.Thr158Ile) | Missense | <0.1% | Unknown functional impact; reported in COSMIC |
| c.682G>A (p.Glu228Lys) | Missense | <0.1% | Potential gain-of-function; observed in breast cancer |
| c.1003C>T (p.Arg335Trp) | Missense | <0.1% | Loss of phosphatase activity in vitro |
Mutation functional classification
Loss of Function (LOF)
Mutations such as p.Arg335Trp reduce catalytic activity, impairing G2/M checkpoint control.
Gain of Function (GOF)
p.Glu228Lys may enhance phosphatase activity, promoting unscheduled cell cycle entry.
Dominant Negative (DN)
No well-characterized dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • protein tyrosine phosphatase activity | • cell cycle |
| • G2/M transition of mitotic cell cycle | • dephosphorylation |
| • mitotic cell cycle checkpoint | • protein dephosphorylation |
Pathways
• Cell Cycle (KEGG hsa04110)
• G2/M DNA Damage Checkpoint (Reactome R-HSA-69481)
• CDK-mediated phosphorylation and removal of Cdc6 (Reactome R-HSA-69017)
Protein Summary
CDC25B is a 580-amino acid dual-specificity phosphatase that removes inhibitory phosphates from CDK1 (Thr14/Tyr15) and CDK2, driving G2/M progression. It contains a N-terminal regulatory domain and a C-terminal catalytic domain. The protein is regulated by phosphorylation, ubiquitination, and subcellular localization. Overexpression is oncogenic, and it is a target for cancer therapeutics.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CDC25B Knockout HEK293 Cell Line | EDJ-KQ633 | Human | 994 | Details Get a Quote |
| CDC25B Knockout A-549 Cell Line | EDJ-KQ19100 | Human | 994 | Details Get a Quote |
| CDC25B Knockout HCT 116 Cell Line | EDJ-KQ19101 | Human | 994 | Details Get a Quote |
| CDC25B Knockout HeLa Cell Line | EDJ-KQ19102 | Human | 994 | Details Get a Quote |
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