BRD3: Bromodomain Containing 3
A chromatin reader protein involved in transcriptional regulation and cancer
Gene Information Card
| Symbol | BRD3 |
|---|---|
| Full Name | Bromodomain Containing 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 9q34.2 |
| NCBI Gene ID | 8019 ncbi.nlm.nih.gov/gene/8019 |
| Ensembl ID | ENSG00000169925 |
| UniProt ID | Q15059 |
| OMIM ID | 601541 |
| HGNC ID | 1103 |
| Aliases | RING3L, ORFX |
Description
BRD3 (Bromodomain Containing 3) is a protein-coding gene that encodes a member of the BET (bromodomain and extra-terminal) family. The protein contains two bromodomains that recognize acetylated lysine residues on histones, facilitating chromatin remodeling and transcriptional activation. BRD3 is involved in cell cycle regulation, gene expression, and has been implicated in cancer, particularly through fusion with NUTM1 in NUT midline carcinoma.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| NUT midline carcinoma | BRD3-NUT fusion protein drives oncogenesis by aberrantly activating transcription of pro-proliferative genes | PMID: 21575861 |
| Cancer (general) | BRD3 overexpression or mutation may contribute to tumorigenesis via dysregulated chromatin binding | PMID: 21575861 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.3 | Medium |
| Bone marrow | 8.7 | Medium |
| Lymph node | 7.5 | Medium |
| Brain | 5.2 | Low |
| Liver | 4.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| K562 (leukemia) | 15.2 | High expression |
| HeLa (cervical cancer) | 10.8 | Medium expression |
| A549 (lung cancer) | 9.3 | Medium expression |
| MCF7 (breast cancer) | 7.1 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| BRD3-NUT fusion | Gene fusion | Rare | Oncogenic; produces a chimeric protein that drives NUT midline carcinoma |
| Missense variants | Missense | Unknown | Potential impact on bromodomain function; not well characterized |
Mutation functional classification
Loss of Function (LOF)
Not commonly reported; loss of BRD3 function may impair normal transcriptional regulation.
Gain of Function (GOF)
BRD3-NUT fusion acts as a gain-of-function oncoprotein by aberrantly activating transcription.
Dominant Negative (DN)
Not documented for BRD3.
View complete mutation data：
Gene Ontology (GO)
| • protein binding (GO:0005515) | • histone binding (GO:0042393) |
| • chromatin binding (GO:0003682) | • regulation of transcription by RNA polymerase II (GO:0006357) |
| • nucleus (GO:0005634) |
Pathways
• Chromatin organization
• Transcriptional regulation by BET proteins
• Cell cycle
Protein Summary
BRD3 is a 726-amino acid protein belonging to the BET family. It contains two N-terminal bromodomains (BD1 and BD2) that bind acetylated histones, and an extra-terminal (ET) domain involved in protein-protein interactions. BRD3 localizes to the nucleus and regulates transcription by recruiting transcriptional complexes to chromatin. It is ubiquitously expressed with higher levels in testis and hematopoietic tissues.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| BRD3 Knockout HEK293 Cell Line | EDJ-KQ5461 | Human | 8019 | Details Get a Quote |
| BRD3OS Knockout HEK293 Cell Line | EDJ-KQ12565 | Human | 266655 | Details Get a Quote |
| BRD3 Knockout A-549 Cell Line | EDJ-KQ29969 | Human | 8019 | Details Get a Quote |
| BRD3 Knockout HCT 116 Cell Line | EDJ-KQ29971 | Human | 8019 | Details Get a Quote |
| BRD3 Knockout HeLa Cell Line | EDJ-KQ29972 | Human | 8019 | Details Get a Quote |
| BRD3OS Knockout A-549 Cell Line | EDJ-KQ41582 | Human | 266655 | Details Get a Quote |
| BRD3OS Knockout HCT 116 Cell Line | EDJ-KQ41583 | Human | 266655 | Details Get a Quote |
| BRD3OS Knockout HeLa Cell Line | EDJ-KQ41584 | Human | 266655 | Details Get a Quote |
