ARAP3: ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 3
A multi-domain GTPase-activating protein involved in cell adhesion, migration, and phosphoinositide signaling.
Gene Information Card
| Symbol | ARAP3 |
|---|---|
| Full Name | ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 5q31.3 |
| NCBI Gene ID | 64411 ncbi.nlm.nih.gov/gene/64411 |
| Ensembl ID | ENSG00000113580 |
| UniProt ID | Q8WWN8 |
| OMIM ID | 606647 |
| HGNC ID | 24058 |
| Aliases | CENTD3, DRAG1, KIAA0702, centaurin-delta-3 |
Description
ARAP3 encodes a multi-domain protein that functions as a GTPase-activating protein (GAP) for both Arf and Rho family small GTPases. It contains an N-terminal ArfGAP domain, a RhoGAP domain, ankyrin repeats, and a pleckstrin homology (PH) domain. ARAP3 is involved in regulating cell adhesion, cytoskeletal dynamics, and cell migration, particularly in response to phosphoinositide 3-kinase (PI3K) signaling. It is widely expressed and plays roles in vascular development and immune cell function.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various types) | Dysregulation of ARAP3 expression and mutations may alter cell migration and invasion through Rho/Arf signaling pathways. | COSMIC database reports somatic mutations in multiple cancer types; limited functional validation. |
| Thrombocytopenia | ARAP3 knockout mice exhibit platelet defects; human relevance not fully established. | OMIM #606647; mouse model studies. |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Lung | 8.3 | Low |
| Heart | 6.1 | Low |
| Liver | 4.2 | Low |
| Kidney | 7.8 | Low |
| Testis | 15.2 | Medium |
| Spleen | 10.1 | Medium |
| Placenta | 9.4 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 11.3 | Embryonic kidney cells |
| HeLa | 9.8 | Cervical cancer cells |
| K562 | 7.2 | Leukemia cells |
| A549 | 8.5 | Lung cancer cells |
| HepG2 | 6.0 | Liver cancer cells |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| p.Gly111Arg | Missense | <0.1% | Unknown; reported in COSMIC |
| p.Arg422* | Nonsense | <0.1% | Predicted loss of function |
| p.Leu567Phe | Missense | <0.1% | Unknown; reported in COSMIC |
Mutation functional classification
Loss of Function (LOF)
Nonsense mutations (e.g., p.Arg422*) likely result in truncated protein and loss of GAP activity.
Gain of Function (GOF)
No well-characterized gain-of-function mutations reported.
Dominant Negative (DN)
Not established for ARAP3.
View complete mutation data：
Gene Ontology (GO)
Pathways
• PI3K-Akt signaling pathway
• Rho GTPase cycle
• Arf GTPase cycle
• Regulation of actin cytoskeleton
• Focal adhesion
Protein Summary
ARAP3 is a 1504-amino acid protein that integrates signals from phosphoinositides and small GTPases. It contains an ArfGAP domain that inactivates Arf6, and a RhoGAP domain that inactivates RhoA and Cdc42. The PH domain binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3), linking ARAP3 to PI3K signaling. Through its GAP activities, ARAP3 modulates cell adhesion, spreading, and migration. It is implicated in vascular development and cancer cell invasion.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| ARAP3 Knockout HEK293 Cell Line | EDJ-KQ1336 | Human | 64411 | Details Get a Quote |
| ARAP3 Knockout A-549 Cell Line | EDJ-KQ20788 | Human | 64411 | Details Get a Quote |
| ARAP3 Knockout HCT 116 Cell Line | EDJ-KQ20789 | Human | 64411 | Details Get a Quote |
| ARAP3 Knockout HeLa Cell Line | EDJ-KQ20790 | Human | 64411 | Details Get a Quote |
