ADORA3: Adenosine A3 Receptor
A key G protein-coupled receptor in purinergic signaling, implicated in inflammation, ischemia, and cancer.
Gene Information Card
| Symbol | ADORA3 |
|---|---|
| Full Name | Adenosine A3 Receptor |
| Gene Type | protein-coding |
| Chromosomal Location | 1p13.2 |
| NCBI Gene ID | 140 ncbi.nlm.nih.gov/gene/140 |
| Ensembl ID | ENSG00000182628 |
| UniProt ID | P0DMS8 |
| OMIM ID | 600445 |
| HGNC ID | 268 |
| Aliases | A3AR, AD026 |
Description
ADORA3 encodes the adenosine A3 receptor, a member of the G protein-coupled receptor (GPCR) family. This receptor binds extracellular adenosine and mediates diverse physiological effects, including inhibition of adenylyl cyclase, modulation of cAMP levels, and activation of phospholipase C. It is involved in inflammatory responses, cardioprotection during ischemia, and tumor growth regulation. Expression is highest in lung, liver, and immune cells.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Asthma | ADORA3 activation on mast cells and eosinophils promotes bronchoconstriction and airway inflammation. | ClinVar, NCBI Gene |
| Myocardial Ischemia | Receptor activation during ischemia-reperfusion reduces infarct size via Gi/o protein signaling. | NCBI Gene, OMIM |
| Rheumatoid Arthritis | ADORA3 overexpression in synovial fibroblasts contributes to chronic inflammation and joint destruction. | NCBI Gene |
| Colorectal Cancer | ADORA3 signaling modulates tumor cell proliferation and apoptosis; expression correlates with poor prognosis. | COSMIC, NCBI Gene |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Lung | 12.5 | Medium |
| Liver | 8.3 | Low |
| Spleen | 15.2 | Medium |
| Whole Blood | 6.1 | Low |
| Testis | 3.4 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 18.7 | High expression in recombinant systems |
| Jurkat | 22.1 | T-cell line; high endogenous expression |
| HepG2 | 9.8 | Hepatocellular carcinoma; moderate expression |
| A549 | 14.3 | Lung adenocarcinoma; moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G | Missense | <0.01% | p.Met1Val; loss of start codon, likely loss-of-function |
| c.154C>T | Missense | <0.01% | p.Arg52Cys; reduced receptor activity |
| c.911G>A | Synonymous | 0.02% | p.Pro304Pro; no amino acid change |
Mutation functional classification
Loss of Function (LOF)
Mutations that impair receptor expression or signaling (e.g., start codon loss, missense variants reducing ligand binding).
Gain of Function (GOF)
Not well-documented; no activating mutations reported in major databases.
Dominant Negative (DN)
Not described for ADORA3.
View complete mutation data：
Gene Ontology (GO)
| • G protein-coupled receptor activity | • adenosine receptor activity |
| • adenylate cyclase inhibiting G protein-coupled receptor signaling pathway | • inflammatory response |
| • positive regulation of MAPK cascade |
Pathways
• Adenosine signaling pathway
• GPCR downstream signaling
• cAMP signaling pathway
Protein Summary
The adenosine A3 receptor is a 318-amino acid integral membrane protein with seven transmembrane domains. It couples primarily to Gi/o proteins, inhibiting adenylyl cyclase and reducing cAMP. The receptor also activates phospholipase C and MAP kinases. It is N-glycosylated and palmitoylated. Expression is regulated by hypoxia and inflammation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| ADORA3 Knockout HEK293 Cell Line | EDJ-KQ50103 | Human | 140 | Details Get a Quote |
| ADORA3 Knockout HeLa Cell Line | EDJ-KQ52564 | Human | 140 | Details Get a Quote |
| ADORA3 Knockout A-549 Cell Line | EDJ-KQ61043 | Human | 140 | Details Get a Quote |
| ADORA3 Knockout HCT 116 Cell Line | EDJ-KQ69520 | Human | 140 | Details Get a Quote |
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