ADAM32 Gene - A Disintegrin and Metalloproteinase Domain 32
Comprehensive genomic and proteomic analysis of ADAM32, a testis-enriched metalloproteinase implicated in spermatogenesis and cancer.
Gene Information Card
| Symbol | ADAM32 |
|---|---|
| Full Name | ADAM metallopeptidase domain 32 |
| Gene Type | protein-coding |
| Chromosomal Location | 8p11.23 |
| NCBI Gene ID | 203102 ncbi.nlm.nih.gov/gene/203102 |
| Ensembl ID | ENSG00000164733 |
| UniProt ID | Q8TC27 |
| OMIM ID | 614266 |
| HGNC ID | 15478 |
| Aliases | ADAM 32, ADAM metallopeptidase domain 32, disintegrin and metalloproteinase domain-containing protein 32 |
Description
ADAM32 (ADAM metallopeptidase domain 32) is a member of the ADAM (a disintegrin and metalloproteinase) family. This gene encodes a transmembrane protein that contains a metalloproteinase domain, a disintegrin domain, a cysteine-rich region, and an epidermal growth factor (EGF)-like domain. ADAM32 is predominantly expressed in the testis and is thought to play a role in spermatogenesis, cell adhesion, and proteolysis. Alternative splicing results in multiple transcript variants.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Lung cancer | ADAM32 mutations (e.g., missense) may alter metalloproteinase activity, potentially affecting cell migration and invasion. | COSMIC database reports somatic mutations in lung adenocarcinoma samples. |
| Colorectal cancer | Somatic missense and nonsense mutations in ADAM32 have been identified in colorectal tumors, though functional impact remains under investigation. | COSMIC database; limited functional validation. |
| Testicular germ cell tumors | Given high testis expression, ADAM32 dysregulation may contribute to germ cell tumorigenesis, but direct evidence is sparse. | NCBI Gene expression data; no definitive mechanism established. |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 38.5 | High |
| Fallopian tube | 3.2 | Low |
| Prostate | 2.1 | Low |
| Thyroid | 1.8 | Low |
| Adrenal gland | 1.5 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| NCI-H1299 (lung carcinoma) | 2.3 | Moderate expression in lung cancer cell line |
| A549 (lung carcinoma) | 1.9 | Low expression |
| MCF7 (breast adenocarcinoma) | 0.8 | Very low expression |
| HEK293 (embryonic kidney) | 0.5 | Very low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234G>A (p.Gly412Arg) | Missense | <0.1% in COSMIC | Unknown; predicted possibly damaging by in silico tools |
| c.567C>T (p.Arg189*) | Nonsense | <0.1% in COSMIC | Likely loss of function due to premature truncation |
| c.890A>G (p.Tyr297Cys) | Missense | <0.1% in COSMIC | Unknown; located in disintegrin domain, may affect adhesion |
Mutation functional classification
Loss of Function (LOF)
Nonsense mutations (e.g., p.Arg189*) likely lead to loss of function via truncated protein.
Gain of Function (GOF)
No gain-of-function mutations have been characterized for ADAM32.
Dominant Negative (DN)
No dominant-negative mutations reported for ADAM32.
View complete mutation data：
Gene Ontology (GO)
| • metallopeptidase activity (GO:0008237) | • zinc ion binding (GO:0008270) |
| • integrin binding (GO:0005178) | • proteolysis (GO:0006508) |
| • cell adhesion (GO:0007155) | • membrane (GO:0016020) |
| • extracellular exosome (GO:0070062) |
Pathways
• ADAM-mediated ectodomain shedding (Reactome: R-HSA-6809371)
• Integrin cell surface interactions (Reactome: R-HSA-216083)
Protein Summary
ADAM32 is a transmembrane metalloproteinase of 789 amino acids (UniProt Q8TC27). It contains a signal peptide, a prodomain, a metalloproteinase domain with a zinc-binding motif (HEXXHXXGXXH), a disintegrin domain, a cysteine-rich domain, and an EGF-like domain. The protein is predicted to function in ectodomain shedding and cell-cell adhesion, particularly in the testis. Its catalytic activity has not been fully characterized experimentally.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| ADAM32 Knockout HEK293 Cell Line | EDJ-KQ4665 | Human | 203102 | Details Get a Quote |
| ADAM32 Knockout A-549 Cell Line | EDJ-KQ28594 | Human | 203102 | Details Get a Quote |
| ADAM32 Knockout HCT 116 Cell Line | EDJ-KQ28596 | Human | 203102 | Details Get a Quote |
| ADAM32 Knockout HeLa Cell Line | EDJ-KQ28597 | Human | 203102 | Details Get a Quote |
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