Contact Us

Validated stable reporter cell lines maintain consistent signal response across multiple passages, making them suitable for quantitative and comparative analysis.

Common systems include Luciferase and fluorescent proteins (e.g., GFP, RFP). The detection method can be chosen based on specific experimental requirements.

Gene interference (gene knockdown) is a technique that partially suppresses the expression of target genes using small RNA molecules (such as siRNA or shRNA) or CRISPRi systems:
siRNA/shRNA: The siRNA associates with the cellular RISC complex to degrade target mRNA, thereby blocking protein translation.
CRISPRi: A modified dCas9 protein binds to a guide RNA (gRNA) and blocks the gene promoter, repressing transcription without cleaving DNA.
Applications: Gene interference is widely used for functional genomics studies, disease mechanism investigation, and drug target validation.

siRNA：	Transient effect (5–7 days),suitable for short-term phenotypic analysis
shRNA/CRISPRi：	Stable knockdown after selection, permanent suppression, effective over 20+ passages

Parameter	siRNA	shRNA (viral vector)	CRISPRi
Duration of Effect	Transient (5-7 days)	Long-term (stably maintained through passages)	Long-term and stable
Cell Type Compatibility	Easily transfected cells	Broad spectrum, including hard-to-transfect cells	Requires pre-established dCas9 cell line
Off-target Risk	Medium to high	Medium	Very low
Typical Use	Rapid validation	Animal or cell models	High-precision gene regulation

Recommendations:
For Rapid Validation: Use siRNA. Suitable for quickly testing the function of 1–2 genes in easily transfected cells. This approach is cost-effective and fast.
For Stable Gene Knockdown in Cells or In Vivo: Use shRNA (viral delivery). A well-established technique that enables long-term and stable gene silencing, widely applied in the construction of cellular and animal models.
For High Specificity, Low Off-target, Reversible Regulation, Multi-gene Manipulation, Non-coding RNA Targeting: Use CRISPRi (viral delivery to establish stable cell lines).

Technology	Applications	Delivery Format
siRNA transfection	Rapid validation (effects observed within 72-96 hours)	Cells post-interference/experimental report
shRNA viral vectors	Long-term suppression (stable cell line screening)	Viral particles/stable knockdown cell lines
CRISPRi	High-specificity gene inhibition	dCas9-expressing cell line and gRNA vector

Both iPSCs and embryonic stem cells (ESCs) have pluripotency, but iPSCs are obtained by reprogramming the somatic cells, while ESCs come from early embryos. IPSC does not involve the use of embryos and does not violate ethical requirements, so it can also avoid immune rejection issues in scientific research. Therefore, iPSC is considered to be a preferred choice.

Induced pluripotent stem cells (iPSCs) are a type of cell that reprogram the somatic cells into a pluripotent state. They have characteristics similar to embryonic stem cells and can differentiate into almost all cell types in the body. Therefore, scientists can use IPSC cells to generate various cell types in vitro for research and treatment, instead of using embryonic stem cells to achieve the experimental purposes.

iPSCs have broad clinical potential, including applications in cell therapy (e.g., for diabetes or heart disease treatment), tissue engineering (e.g., development of artificial skin or liver tissue), and personalized drug screening (e.g., selecting optimal treatments based on a patient’s specific cellular response). These applications may transform treatment methods, offering more effective and personalized medical services.

Yes, suitable for automated screening platforms.

Gene knock-in technology involves inserting an exogenous gene sequence into a specific location within the genome for gene function studies. Edigene utilizes advanced gene editing tools, such as the CRISPR/Cas9 system, to guide nucleases to cut the target DNA, and employs homology-directed repair or non-homologous end joining to accurately insert the gene at the desired location, achieving efficient and precise gene knock-in.

EDITGENE brings 10 years of CRISPR-based cell editing experience and offers one-on-one support from a team of PhDs from globally recognized institutions.

Gene overexpression aids in studying the function of specific genes, revealing their role within the organism. It is also commonly used in drug screening, vaccine development, and protein production. For example, by overexpressing a therapeutic protein, researchers can evaluate its efficacy in disease models.

Gene overexpression refers to using various techniques to significantly increase the expression level of a specific gene in cells or organisms. This is often achieved by introducing additional gene copies or using strong promoters to drive gene expression.

Monoclonal screening is the process of isolating a single clone from a mixed pool of cells and expanding that clone into a cell line. Monoclonal screening ensures that the cell lines used originate from a single cell, guaranteeing a high degree of genetic background consistency. After cells are gene-edited or genetically modified, the genetic background differences among the cells in the initial cell pool can be significant, making subsequent experimental results inaccurate. By using monoclonal screening, researchers can obtain cell populations with consistent genetic backgrounds and stable gene edits, allowing for stable and accurate monitoring of phenotypic changes.