Contact Us

It is recommended to conduct a pre-transfection experiment before the main experiment. Try different transfection methods to find optimal conditions, such as common chemical transfection methods (e.g., lipofection) or physical methods (e.g., electroporation).

The Bingo™ PE, pegRNA, and gRNA in the kit are all plasmids, compatible with both lipofection and electroporation. You can choose the appropriate transfection method and parameters based on cell type. Generally, adherent cells can be transfected by either method, while therapeutically relevant cells are recommended to use electroporation

Gene mutations may affect cell viability. Before conducting a gene point mutation experiment, it is advisable to consult relevant literature to understand the function of the target gene. If the target gene plays a critical role in cell proliferation or survival, point mutations may hinder these processes, making it difficult to obtain positive cells.

The kit’s Positive Control has been validated in HeLa cells (human gene) and N2a cells (mouse gene). Due to the high heterogeneity of cells, transfection and editing efficiency may vary in different cell types using the same kit.

Yes. The Bingo™ CRISPR Point Mutation Cell Line Generation Kit targets genomic DNA for gene mutation, allowing the genotype of the target cells to be stably inherited by subsequent generations.

For most cell lines, Mycoplasma elimination does not affect cell health. However, for sensitive cells, it is recommended to back up the cells before treatment. If cell death or poor conditions occur, reduce the working concentration by half and extend the treatment cycle to 28 days.

In most cases, mycoplasma should be completely eliminated after treatment. If residual contamination persists, increase the working concentration by 50% and repeat the treatment for another cycle.

Since this reagent already provides antibiotic functionality, additional antibiotics are not recommended during the treatment to avoid adding unnecessary stress to the cells.

No, simultaneous use of both reagents places excessive stress on cells and increases the likelihood of resistance development. Use the reagents sequentially according to the instructions.

The PB system in this kit includes plasmids compatible with lipofection, electroporation, or other transfection methods. You can select the transfection method and parameters based on cell type. Most adherent cells are compatible with various transfection methods, while electroporation is recommended for therapeutically relevant cells.

Yes. The piggyBac Transposon System Kit targets genomic DNA for gene transposition, allowing the genotype of target cells to be stably inherited. Note: Maintain transposed cells with half-dose puromycin for stability.

The transposon plasmid in this kit contains the CopGFP gene. Successfully transposed cells will exhibit green fluorescence under a fluorescence microscope. Due to cell heterogeneity, transfection and transposition efficiencies may vary across cell types. To minimize transposition variability, optimize transfection methods for target cells.

Since this reagent already provides antibiotic functions, additional antibiotics are not recommended during the treatment to reduce stress on cells.

Mycoplasma should be nearly completely eliminated. If contamination persists, increase the working concentration by 50% and repeat the treatment for one cycle.

The choice depends on whether you are studying iron uptake biology, transferrin endocytosis, or using TFRC as a model receptor for clathrin-mediated endocytosis research. The Knockout line is the standard tool for asking whether TFRC is required for transferrin-mediated iron uptake — TFRC is the principal iron uptake receptor in most cell types and is essential for proliferating cells. Overexpression is useful for studying TFRC trafficking dynamics or for boosting iron uptake capacity. Important consideration: TFRC is essential in most proliferating cells — complete knockout may significantly impair viability and proliferation. The EDITGENE Knockout in HEK293 may exhibit reduced growth that should be characterized before extended use. Rescue with wild-type or endocytosis-deficient (cytoplasmic tail mutant) TFRC enables dissection of iron uptake from endocytic membrane trafficking functions. TFRC is also being explored as an antibody-drug conjugate target — the knockout serves as a critical specificity control for anti-TFRC therapeutics.