KRAS (p.Q61H) Point Mutation in HCT 116 Cell Line

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KRAS (p.Q61H) Point Mutation in HCT 116 Cell Line
Cat.No.:

EDC03023

Species:

Human

Cell Name:

HCT 116

Gene:

KRAS

Gene ID:

3845

Size:

1×10⁶cells

The KRAS (p.Q61H) Point Mutation in HCT116 Cell Line is based on the newly developed Bingo™ platform, which utilizes an optimized and upgraded version of the Prime Editing (PE) gene point mutation system-currently the most efficient and safest technology available. This platform enables precise and efficient gene point mutation, resulting in a highly active and stable cell line.
Cat.No. EDC03023
Product Name KRAS (p.Q61H) Point Mutation in HCT 116 Cell Line
Gene ID
Cell Line HCT 116
Mutation Site p.Q61H
Cellosaurus ID CVCL_0291
Cell Line Synonyms HCT-116, HCT.116, HCT_116, HCT116, HCT116wt, HCT-116/P, HCT-116/parental, CoCL2
Summary
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Associated Diseases Colorectal Carcinoma
Passage Ratio 1:4~1:5
Complete Culture Medium mcCoy5A+10% FBS
Freezing Medium 90% FBS/complete culture medium+10% DMSO
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
LociSTR Info (Sample Cell)
Sample Cell Line: HCT 116		STR Info (Cell bank)
Cell Line: HCT 116
Allele1Allele2Allele3Allele4Allele1Allele2Allele3Allele4
Amelogenin X X
CSF1PO 7 10 7 9 10 11
D2S1338 16 16
D3S1358 12 17 18 19 12 18 19
D5S818 10 11 10 11
D7S820 11 12 11 12
D8S1179 10 12 14 15 10 12 14 15
D13S317 10 12 10 12
D16S539 11 13 11 12 13 14
D18S51 16 17 16 17
D19S433 12 13 12
D21S11 29 30 29 30
FGA 18 23 18 23
Penta D 9 13 9 13
Penta E 12 13 14 12 13 14
TH01 8 9 8 9
TPOX 8 8
vWA 17 21 22 23 17 21 22 23
D6S1043 13
D12S391 17 21 22
D2S441 11 12
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.

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