TSPAN8
Tetraspanin-8: A transmembrane protein involved in cell motility, tumor progression, and exosome biology
Gene Information Card
| Symbol | TSPAN8 |
|---|---|
| Full Name | Tetraspanin 8 |
| Gene Type | Protein coding |
| Chromosomal Location | 12q21.1 |
| NCBI Gene ID | 7103 ncbi.nlm.nih.gov/gene/7103 |
| Ensembl ID | ENSG00000127324 |
| UniProt ID | P19075 |
| OMIM ID | 600769 |
| HGNC ID | 11842 |
| Aliases | CO-029, TM4SF3, TSPAN-8 |
Description
TSPAN8 encodes tetraspanin-8, a member of the tetraspanin family of transmembrane proteins. Tetraspanins organize membrane microdomains and regulate cell adhesion, migration, and signal transduction. TSPAN8 is overexpressed in various cancers and is implicated in tumor progression, metastasis, and exosome-mediated intercellular communication.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Pancreatic cancer | Overexpression promotes invasion and metastasis via integrin signaling and exosome transfer | PMID: 25986610; COSMIC |
| Colorectal cancer | Upregulation correlates with poor prognosis and enhanced cell motility | PMID: 24986688; COSMIC |
| Hepatocellular carcinoma | TSPAN8 expression associated with tumor aggressiveness and EMT | PMID: 27121311; COSMIC |
| Breast cancer | High expression linked to metastasis and exosome-mediated angiogenesis | PMID: 24986688; COSMIC |
| Esophageal squamous cell carcinoma | Amplification and overexpression contribute to tumor progression | PMID: 19137010; COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 12.5 | Medium |
| Pancreas | 8.3 | Low |
| Colon | 6.7 | Low |
| Breast | 4.2 | Low |
| Esophagus | 3.9 | Low |
| Lung | 2.1 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| PANC-1 (pancreatic cancer) | 15.2 | High expression |
| HCT116 (colorectal cancer) | 10.8 | Moderate expression |
| HepG2 (hepatocellular carcinoma) | 9.5 | Moderate expression |
| MCF7 (breast cancer) | 3.1 | Low expression |
| A549 (lung cancer) | 1.8 | Not detected |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.365A>G (p.Asn122Ser) | Missense | <0.1% (COSMIC) | Unknown functional impact |
| c.487C>T (p.Arg163Trp) | Missense | <0.1% (COSMIC) | Unknown functional impact |
| Amplification (12q21.1) | Copy number gain | ~5% in esophageal SCC (COSMIC) | Potential gain of function |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in TSPAN8.
Gain of Function (GOF)
Gene amplification and overexpression observed in multiple cancers suggest a gain-of-function role in tumor progression.
Dominant Negative (DN)
No dominant-negative mutations described for TSPAN8.
View complete mutation data：
Gene Ontology (GO)
| • plasma membrane (GO:0005886) | • integral component of plasma membrane (GO:0005887) |
| • cell adhesion (GO:0007155) | • integral component of membrane (GO:0016021) |
| • cell junction (GO:0030054) | • receptor complex (GO:0043235) |
| • extracellular exosome (GO:0070062) |
Pathways
• Tetraspanin-enriched microdomains (TEMs)
• Integrin signaling pathway
• Exosome biogenesis and secretion
• Cell adhesion and migration
Protein Summary
Tetraspanin-8 is a 4-transmembrane domain protein (237 amino acids) localized to the plasma membrane and exosomes. It forms complexes with integrins and other tetraspanins, modulating cell adhesion, migration, and signal transduction. Overexpression in cancers enhances tumor cell motility, invasion, and metastasis. TSPAN8 is also a component of exosomes, facilitating intercellular transfer of oncogenic signals.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TSPAN8 Knockout HEK293 Cell Line | EDJ-KQ3919 | Human | 7103 | Details Get a Quote |
| TSPAN8 Knockout HCT 116 Cell Line | EDJ-KQ26146 | Human | 7103 | Details Get a Quote |
| TSPAN8 Knockout HeLa Cell Line | EDJ-KQ26147 | Human | 7103 | Details Get a Quote |
| TSPAN8 Knockout A-549 Cell Line | EDJ-KQ63152 | Human | 7103 | Details Get a Quote |
