TRIM71: A Key Regulator of Stem Cell Differentiation and Cancer
Comprehensive gene card for TRIM71, including expression, mutations, and disease associations.
Gene Information Card
| Symbol | TRIM71 |
|---|---|
| Full Name | Tripartite Motif Containing 71 |
| Gene Type | Protein coding |
| Chromosomal Location | 3p22.3 |
| NCBI Gene ID | 131405 ncbi.nlm.nih.gov/gene/131405 |
| Ensembl ID | ENSG00000106565 |
| UniProt ID | Q2Q1W2 |
| OMIM ID | 618570 |
| HGNC ID | 16231 |
| Aliases | LIN41, LIN-41 homolog |
Description
TRIM71 (tripartite motif containing 71) is a member of the TRIM-NHL protein family. It functions as an E3 ubiquitin ligase and a post-transcriptional regulator of gene expression, primarily by binding to mRNA targets and repressing translation. TRIM71 is essential for stem cell self-renewal and differentiation, particularly in neural and germ cell lineages. It is also implicated in cancer, where it can act as an oncogene or tumor suppressor depending on the cellular context.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Germ cell tumors | TRIM71 overexpression promotes tumor growth by repressing tumor suppressor mRNAs | PMID: 25686878 |
| Neuroblastoma | TRIM71 is amplified and drives oncogenesis through LIN28/let-7 pathway disruption | PMID: 29622468 |
| Hepatocellular carcinoma | TRIM71 upregulation correlates with poor prognosis and promotes metastasis | PMID: 31073040 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Brain | 8.3 | Medium |
| Ovary | 6.1 | Low |
| Liver | 2.4 | Low |
| Heart | 1.1 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| NTERA-2 (embryonal carcinoma) | 15.2 | High expression |
| SH-SY5Y (neuroblastoma) | 9.8 | Moderate expression |
| HepG2 (hepatocellular carcinoma) | 7.4 | Moderate expression |
| HEK293 (embryonic kidney) | 3.1 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T (p.Arg412Cys) | Missense | <0.1% | Altered RNA binding affinity |
| c.567_568insA (p.Glu190Argfs*5) | Frameshift | <0.1% | Loss of function |
| c.2002G>A (p.Glu668Lys) | Missense | <0.1% | Unknown |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations that truncate the NHL domain or RING finger domain lead to loss of E3 ligase activity and RNA binding.
Gain of Function (GOF)
Missense mutations in the NHL domain may enhance mRNA target binding, leading to increased repression of tumor suppressors.
Dominant Negative (DN)
Mutations that disrupt dimerization or substrate recognition without complete loss of function may act in a dominant-negative manner.
View complete mutation data：
Gene Ontology (GO)
| • RNA binding | • ubiquitin protein ligase activity |
| • regulation of translation | • stem cell population maintenance |
| • negative regulation of miRNA-mediated gene silencing |
Pathways
• LIN28/let-7 signaling pathway
• p53 pathway
• Wnt signaling pathway
Protein Summary
TRIM71 is a 931-amino acid protein containing an N-terminal RING finger domain (E3 ubiquitin ligase activity), two B-box domains, a coiled-coil region, and a C-terminal NHL domain (RNA binding). It ubiquitinates target proteins such as LIN28 and AGO2, and binds to the 3' UTR of mRNAs like CDKN1A and EGR1 to repress translation. TRIM71 is critical for maintaining pluripotency and regulating differentiation in embryonic stem cells.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TRIM71 Knockout HEK293 Cell Line | EDJ-KQ9267 | Human | 131405 | Details Get a Quote |
| TRIM71 Knockout HeLa Cell Line | EDJ-KQ58296 | Human | 131405 | Details Get a Quote |
| TRIM71 Knockout A-549 Cell Line | EDJ-KQ66784 | Human | 131405 | Details Get a Quote |
| TRIM71 Knockout HCT 116 Cell Line | EDJ-KQ75187 | Human | 131405 | Details Get a Quote |
