TRIM35
Tripartite Motif Containing 35
Gene Information Card
| Symbol | TRIM35 |
|---|---|
| Full Name | Tripartite Motif Containing 35 |
| Gene Type | Protein coding |
| Chromosomal Location | 8p21.2 |
| NCBI Gene ID | 23087 ncbi.nlm.nih.gov/gene/23087 |
| Ensembl ID | ENSG00000104219 |
| UniProt ID | Q9UPQ4 |
| OMIM ID | 616144 |
| HGNC ID | 16285 |
| Aliases | HLS5, MAIR, RNF33 |
Description
TRIM35 (tripartite motif containing 35) encodes a member of the tripartite motif (TRIM) family, characterized by a RING finger domain, B-box, and coiled-coil region. The protein functions as an E3 ubiquitin ligase involved in innate immunity, cell cycle regulation, and apoptosis. It is known to interact with and regulate p53 stability, and has been implicated in tumor suppression, particularly in hepatocellular carcinoma and breast cancer.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | TRIM35 acts as a tumor suppressor by promoting p53 ubiquitination and degradation, leading to altered cell cycle control. Loss of TRIM35 expression correlates with poor prognosis. | PMID: 23431138 |
| Breast cancer | Reduced TRIM35 expression is associated with increased proliferation and metastasis. TRIM35 modulates the PI3K/AKT pathway. | PMID: 27134165 |
| Innate immune response | TRIM35 negatively regulates RIG-I-like receptor signaling by ubiquitinating MAVS, thereby limiting antiviral responses. | PMID: 22105175 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 12.5 | Medium |
| Breast | 8.3 | Low |
| Lung | 6.7 | Low |
| Spleen | 15.2 | Medium |
| Testis | 18.9 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 14.1 | Hepatocellular carcinoma cell line |
| MCF7 | 9.8 | Breast cancer cell line |
| A549 | 7.5 | Lung cancer cell line |
| HEK293 | 11.3 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Pro34Leu) | Missense | <0.01% | Unknown functional effect; reported in COSMIC |
| c.457G>A (p.Glu153Lys) | Missense | <0.01% | Reported in COSMIC; potential loss of function |
| c.832C>T (p.Arg278Trp) | Missense | <0.01% | Reported in COSMIC; located in coiled-coil domain |
Mutation functional classification
Loss of Function (LOF)
Missense mutations in the RING domain may impair E3 ubiquitin ligase activity, reducing p53 regulation and promoting tumorigenesis.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No confirmed dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Ubiquitin mediated proteolysis (KEGG: hsa04120)
• p53 signaling pathway (KEGG: hsa04115)
• RIG-I-like receptor signaling pathway (KEGG: hsa04622)
Protein Summary
TRIM35 is a 493-amino acid protein with a molecular weight of approximately 56 kDa. It contains an N-terminal RING finger domain (required for E3 ubiquitin ligase activity), two B-box domains, a coiled-coil region, and a C-terminal PRY/SPRY domain. The protein localizes to both cytoplasm and nucleus. TRIM35 ubiquitinates p53, promoting its degradation, and also targets MAVS to negatively regulate antiviral signaling. Its expression is frequently downregulated in hepatocellular carcinoma and breast cancer, suggesting a tumor suppressor role.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TRIM35 Knockout HEK293 Cell Line | EDJ-KQ2945 | Human | 23087 | Details Get a Quote |
| TRIM35 Knockout A-549 Cell Line | EDJ-KQ24069 | Human | 23087 | Details Get a Quote |
| TRIM35 Knockout HeLa Cell Line | EDJ-KQ24071 | Human | 23087 | Details Get a Quote |
| TRIM35 Knockout HCT 116 Cell Line | EDJ-KQ22701 | Human | 23087 | Details Get a Quote |
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