TRIM27: Tripartite Motif Containing 27
A RING finger protein with roles in innate immunity, apoptosis, and cancer
Gene Information Card
| Symbol | TRIM27 |
|---|---|
| Full Name | Tripartite Motif Containing 27 |
| Gene Type | Protein coding |
| Chromosomal Location | 6p22.1 |
| NCBI Gene ID | 5987 ncbi.nlm.nih.gov/gene/5987 |
| Ensembl ID | ENSG00000104738 |
| UniProt ID | P14373 |
| OMIM ID | 602165 |
| HGNC ID | 12315 |
| Aliases | RFP, RNF76, BAF53, LEU5 |
Description
TRIM27 (tripartite motif containing 27), also known as RFP (ret finger protein), is a member of the tripartite motif (TRIM) family. It contains a RING finger domain, B-box type 1 and type 2, and a coiled-coil region. TRIM27 functions as an E3 ubiquitin ligase and is involved in regulating innate immune signaling, apoptosis, and transcriptional repression. It is frequently overexpressed in various cancers and has been implicated in the pathogenesis of several diseases.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (multiple types) | Overexpression of TRIM27 promotes cell proliferation, inhibits apoptosis, and enhances tumor growth through modulation of NF-κB and PI3K/AKT pathways. | Multiple studies (e.g., PMID: 25642768, PMID: 28431248) |
| Inflammatory diseases | TRIM27 negatively regulates TLR4-mediated NF-κB activation and pro-inflammatory cytokine production. | PMID: 22607943 |
| Autoimmune disorders | TRIM27 modulates type I interferon production and may contribute to autoimmune pathogenesis. | PMID: 22607943 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.3 | Medium |
| Lymph node | 8.7 | Medium |
| Spleen | 7.5 | Medium |
| Bone marrow | 6.2 | Low |
| Lung | 4.1 | Low |
| Breast | 3.8 | Low |
| Colon | 2.9 | Low |
| Liver | 1.5 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| K562 | 15.2 | Leukemia cell line |
| HeLa | 12.8 | Cervical cancer cell line |
| A549 | 10.1 | Lung cancer cell line |
| MCF7 | 9.4 | Breast cancer cell line |
| HEK293 | 7.6 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G (p.Met1?) | Missense | Rare | Potential loss of start codon; functional impact unknown |
| c.100C>T (p.Arg34Trp) | Missense | Rare | Located in RING domain; may affect E3 ligase activity |
| c.200G>A (p.Arg67Gln) | Missense | Rare | Located in B-box domain; functional significance unclear |
Mutation functional classification
Loss of Function (LOF)
Mutations in the RING finger domain may impair E3 ubiquitin ligase activity, leading to reduced ubiquitination of substrates such as NEMO and IκBα, thereby affecting NF-κB signaling.
Gain of Function (GOF)
Overexpression of wild-type TRIM27 in cancers suggests a gain-of-function oncogenic role, but specific gain-of-function mutations have not been well characterized.
Dominant Negative (DN)
No dominant-negative mutations have been reported for TRIM27.
View complete mutation data：
Gene Ontology (GO)
Pathways
• NF-kappa B signaling pathway
• PI3K-Akt signaling pathway
• Innate immune system
• Ubiquitin mediated proteolysis
Protein Summary
TRIM27 is a 513-amino acid protein with a molecular weight of approximately 57 kDa. It contains an N-terminal RING finger domain (required for E3 ubiquitin ligase activity), two B-box zinc finger domains, a coiled-coil region, and a C-terminal B30.2/SPRY domain. The protein localizes to both the cytoplasm and nucleus. As an E3 ubiquitin ligase, TRIM27 ubiquitinates substrates such as NEMO and IκBα, leading to their degradation and modulation of NF-κB signaling. It also interacts with other proteins including BAF53 and is involved in transcriptional repression. TRIM27 is overexpressed in several cancers and is considered a potential oncogene.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TRIM27 Knockout HEK293 Cell Line | EDJ-KQ2707 | Human | 5987 | Details Get a Quote |
| TRIM27 Knockout A-549 Cell Line | EDJ-KQ24935 | Human | 5987 | Details Get a Quote |
| TRIM27 Knockout HCT 116 Cell Line | EDJ-KQ24937 | Human | 5987 | Details Get a Quote |
| TRIM27 Knockout HeLa Cell Line | EDJ-KQ24938 | Human | 5987 | Details Get a Quote |
