TNRC6A Gene
Trinucleotide Repeat Containing Adaptor 6A
Gene Information Card
| Symbol | TNRC6A |
|---|---|
| Full Name | Trinucleotide Repeat Containing Adaptor 6A |
| Gene Type | Protein coding |
| Chromosomal Location | 16p11.2 |
| NCBI Gene ID | 27327 ncbi.nlm.nih.gov/gene/27327 |
| Ensembl ID | ENSG00000103174 |
| UniProt ID | Q8NDV7 |
| OMIM ID | 610739 |
| HGNC ID | 11973 |
| Aliases | CAGH26, GW182, TNRC6A |
Description
TNRC6A (Trinucleotide Repeat Containing Adaptor 6A) encodes a protein that is a key component of the miRNA-induced silencing complex (miRISC). It functions as an adaptor that bridges Argonaute proteins to downstream silencing machinery, facilitating mRNA deadenylation, translational repression, and decay. The protein localizes to cytoplasmic processing bodies (P-bodies) and contains multiple GW repeats essential for interaction with Argonaute. TNRC6A is involved in post-transcriptional gene regulation and has been implicated in various cancers and neurological disorders.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | TNRC6A overexpression correlates with poor prognosis; may promote tumor growth via miRNA pathway dysregulation | PMID: 31570863 |
| Breast cancer | Altered TNRC6A expression linked to miRNA processing defects and metastasis | PMID: 25691885 |
| Neurodevelopmental disorders | Rare variants in TNRC6A associated with intellectual disability and autism spectrum disorder | PMID: 30595370 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 38.2 | High |
| Brain | 25.6 | Medium |
| Liver | 18.4 | Medium |
| Heart | 12.1 | Low |
| Lung | 9.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 32.5 | Embryonic kidney; high expression |
| HeLa | 28.1 | Cervical carcinoma; moderate expression |
| HepG2 | 22.3 | Hepatocellular carcinoma; moderate expression |
| MCF7 | 15.7 | Breast cancer; low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T (p.Arg412*) | Nonsense | <0.01% | Loss of function; truncated protein |
| c.567G>A (p.Trp189*) | Nonsense | <0.01% | Loss of function; premature stop |
| c.890A>G (p.Tyr297Cys) | Missense | <0.01% | Unknown; rare variant in neurodevelopmental cases |
Mutation functional classification
Loss of Function (LOF)
Nonsense mutations (e.g., p.Arg412*, p.Trp189*) lead to truncated protein lacking GW repeats, impairing Argonaute binding and miRNA silencing.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No evidence for dominant-negative effects.
View complete mutation data：
Gene Ontology (GO)
| • miRNA-mediated gene silencing | • P-body assembly |
| • mRNA deadenylation | • translational repression |
| • protein binding | • RNA binding |
Pathways
• miRNA biogenesis and RISC assembly
• mRNA surveillance pathway
• Gene silencing by RNA
Protein Summary
The TNRC6A protein (GW182) is a 1962-amino acid scaffold that contains an N-terminal domain with multiple GW repeats, a ubiquitin-associated domain, and a C-terminal region. It interacts directly with Argonaute proteins via GW repeats and recruits deadenylase complexes (CCR4-NOT) to promote mRNA decay. The protein is predominantly cytoplasmic and localizes to P-bodies. Its structure enables it to function as a central hub in miRNA-mediated gene regulation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TNRC6A Knockout HEK293 Cell Line | EDJ-KQ8762 | Human | 27327 | Details Get a Quote |
| TNRC6A Knockout A-549 Cell Line | EDJ-KQ35023 | Human | 27327 | Details Get a Quote |
| TNRC6A Knockout HCT 116 Cell Line | EDJ-KQ35024 | Human | 27327 | Details Get a Quote |
| TNRC6A Knockout HeLa Cell Line | EDJ-KQ35025 | Human | 27327 | Details Get a Quote |
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