TADA3 Transcriptional Adaptor 3
A component of the SAGA and ATAC histone acetyltransferase complexes involved in transcription regulation and DNA repair
Gene Information Card
| Symbol | TADA3 |
|---|---|
| Full Name | Transcriptional Adaptor 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 3p25.3 |
| NCBI Gene ID | 10474 ncbi.nlm.nih.gov/gene/10474 |
| Ensembl ID | ENSG00000121005 |
| UniProt ID | O75528 |
| OMIM ID | 602946 |
| HGNC ID | 11525 |
| Aliases | ADA3, hADA3, STAF54 |
Description
TADA3 (Transcriptional Adaptor 3) encodes a protein that is a core component of the SAGA (Spt-Ada-Gcn5-acetyltransferase) and ATAC (Ada-Two-A-containing) histone acetyltransferase complexes. These complexes acetylate histones and other proteins, facilitating transcription activation, DNA repair, and cell cycle progression. TADA3 interacts with transcription factors such as p53 and is involved in regulating gene expression, cellular differentiation, and apoptosis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | TADA3 overexpression or mutation may alter p53-mediated transcription and cell cycle control | PMID: 15601826 |
| Colorectal cancer | Altered TADA3 expression linked to dysregulated histone acetylation and tumor progression | PMID: 21516121 |
| Ovarian cancer | TADA3 amplification or mutation affects SAGA complex function and DNA repair | PMID: 22955915 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 15.2 | Medium |
| Brain | 10.5 | Medium |
| Lung | 8.3 | Low |
| Liver | 6.1 | Low |
| Kidney | 7.4 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 12.1 | Cervical cancer cell line |
| HEK293 | 9.8 | Embryonic kidney cell line |
| MCF7 | 11.5 | Breast cancer cell line |
| HCT116 | 10.2 | Colorectal cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1A>G | Missense | <0.1% | Potential loss of function |
| c.200C>T | Nonsense | <0.1% | Truncation, likely loss of function |
| c.350G>A | Missense | <0.1% | Unknown effect |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations in TADA3 are predicted to cause loss of function, impairing SAGA complex integrity and histone acetylation.
Gain of Function (GOF)
No gain-of-function mutations have been reported for TADA3.
Dominant Negative (DN)
Some missense mutations may exert dominant-negative effects by disrupting complex assembly, though evidence is limited.
View complete mutation data：
Gene Ontology (GO)
| • histone acetyltransferase activity | • transcription coactivator activity |
| • chromatin binding | • protein binding |
| • nucleus | • cytoplasm |
| • regulation of transcription by RNA polymerase II | • DNA repair |
| • cell cycle |
Pathways
• SAGA complex
• ATAC complex
• p53 signaling pathway
• Chromatin modifying enzymes
Protein Summary
TADA3 is a 42 kDa protein that contains a conserved Ada3 domain. It serves as an adaptor within the SAGA and ATAC complexes, bridging histone acetyltransferase GCN5/PCAF to other subunits. TADA3 is essential for complex stability and substrate recognition. It also directly interacts with p53, enhancing its transcriptional activity and promoting apoptosis in response to DNA damage.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| TADA3 Knockout HEK293 Cell Line | EDJ-KQ50974 | Human | 10474 | Details Get a Quote |
| TADA3 Knockout HeLa Cell Line | EDJ-KQ55414 | Human | 10474 | Details Get a Quote |
| TADA3 Knockout A-549 Cell Line | EDJ-KQ63894 | Human | 10474 | Details Get a Quote |
| TADA3 Knockout HCT 116 Cell Line | EDJ-KQ72353 | Human | 10474 | Details Get a Quote |
