STK35: Serine/Threonine Kinase 35
A pseudokinase with potential roles in cell cycle regulation and cancer
Gene Information Card
| Symbol | STK35 |
|---|---|
| Full Name | Serine/Threonine Kinase 35 |
| Gene Type | Protein coding (pseudokinase) |
| Chromosomal Location | 20p13 |
| NCBI Gene ID | 140901 ncbi.nlm.nih.gov/gene/140901 |
| Ensembl ID | ENSG00000125845 |
| UniProt ID | Q8TDR2 |
| OMIM ID | 609370 |
| HGNC ID | 29417 |
| Aliases | CLIK1, STK35L1 |
Description
STK35 (serine/threonine kinase 35) encodes a protein belonging to the serine/threonine kinase family. Although it contains a kinase domain, it is considered a pseudokinase due to the absence of key catalytic residues. STK35 is involved in cell cycle regulation, particularly in mitotic progression, and has been implicated in cancer biology through its interaction with the CLIK1 (Cdc2-like kinase 1) pathway. Expression is observed in multiple tissues, with highest levels in testis and certain cancer cell lines.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (general) | Dysregulation of STK35 expression may contribute to tumorigenesis via altered cell cycle control. | COSMIC; literature (PMID: 21779513) |
| Colorectal cancer | STK35 overexpression observed in colorectal tumors; potential role in proliferation. | COSMIC; literature (PMID: 21779513) |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.3 | Medium |
| Bone marrow | 5.1 | Low |
| Lymph node | 4.8 | Low |
| Brain | 2.0 | Low |
| Liver | 1.5 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 8.7 | Cervical cancer cell line |
| HEK293 | 6.2 | Embryonic kidney cells |
| K562 | 4.5 | Leukemia cell line |
| MCF7 | 3.1 | Breast cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Thr34Met) | Missense | <0.01% | Unknown; rare variant in population databases |
| c.452G>A (p.Arg151Gln) | Missense | <0.01% | Unknown; rare variant in population databases |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in ClinVar or COSMIC.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No evidence for dominant-negative effects.
View complete mutation data：
Gene Ontology (GO)
| • protein kinase activity (GO:0004672) | • ATP binding (GO:0005524) |
| • protein phosphorylation (GO:0006468) | • cell cycle (GO:0007049) |
| • mitotic cell cycle (GO:0000278) |
Pathways
• Cell cycle (Reactome: R-HSA-1640170)
• Mitotic G1 phase and G1/S transition (Reactome: R-HSA-453279)
Protein Summary
STK35 is a 433-amino acid protein with a predicted molecular weight of ~48 kDa. It contains a serine/threonine kinase domain but lacks critical catalytic residues (e.g., Lys in the ATP-binding site), classifying it as a pseudokinase. The protein localizes to the nucleus and cytoplasm and interacts with CLIK1, influencing cell cycle progression. Expression is enriched in testis and certain cancer cell lines, suggesting a role in proliferation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| STK35 Knockout HEK293 Cell Line | EDJ-KQ2838 | Human | 140901 | Details Get a Quote |
| STK35 Knockout HeLa Cell Line | EDJ-KQ22461 | Human | 140901 | Details Get a Quote |
| STK35 Knockout A-549 Cell Line | EDJ-KQ23826 | Human | 140901 | Details Get a Quote |
| STK35 Knockout HCT 116 Cell Line | EDJ-KQ23827 | Human | 140901 | Details Get a Quote |
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