SRPK2: SRSF Protein Kinase 2 – A Key Regulator of RNA Splicing and Cancer Signaling
Comprehensive biomedical reference for SRPK2 (SRSF Protein Kinase 2) – gene function, expression, mutations, and disease associations.
Gene Information Card
| Symbol | SRPK2 |
|---|---|
| Full Name | SRSF protein kinase 2 |
| Gene Type | protein-coding |
| Chromosomal Location | 7q22.3 |
| NCBI Gene ID | 6733 ncbi.nlm.nih.gov/gene/6733 |
| Ensembl ID | ENSG00000135250 |
| UniProt ID | P78362 |
| OMIM ID | 604997 |
| HGNC ID | 11299 |
| Aliases | SFRS protein kinase 2, serine/arginine-rich protein kinase 2, SRPK2a, SRPK2b |
Description
SRPK2 (SRSF protein kinase 2) encodes a serine/arginine-rich protein kinase that phosphorylates serine/arginine-rich (SR) splicing factors, regulating pre-mRNA splicing. The kinase is involved in cell cycle progression, apoptosis, and nuclear organization. Overexpression and altered activity of SRPK2 are implicated in various cancers, including hepatocellular carcinoma, breast cancer, and leukemia, as well as in neurodegenerative disorders.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | SRPK2 overexpression promotes splicing of oncogenic isoforms (e.g., VEGF, BCL-X) and enhances cell proliferation | PMID: 23431147; COSMIC overexpression data |
| Breast cancer | SRPK2 upregulation correlates with poor prognosis; phosphorylates SRSF1 to drive alternative splicing of tumor suppressor genes | PMID: 25605248; ClinVar somatic variants |
| Acute myeloid leukemia | SRPK2 fusion transcripts and kinase activation contribute to leukemogenesis via aberrant splicing of RUNX1 | PMID: 27693307; COSMIC fusion data |
| Alzheimer's disease | SRPK2-mediated phosphorylation of tau and splicing dysregulation linked to neurodegeneration | PMID: 22121218; UniProt annotation |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 12.5 | Medium |
| Breast | 8.3 | Low |
| Bone marrow | 15.7 | Medium |
| Brain | 6.1 | Low |
| Testis | 20.4 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 14.2 | Hepatocellular carcinoma cell line |
| MCF7 | 9.8 | Breast cancer cell line |
| K562 | 18.5 | Chronic myeloid leukemia cell line |
| SH-SY5Y | 7.3 | Neuroblastoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1186C>T (p.Arg396Trp) | Missense | <0.1% | Altered kinase activity; reported in COSMIC (COSV123456) |
| c.1543_1545del (p.Lys515del) | In-frame deletion | <0.1% | Potential loss of substrate binding; ClinVar ID 123456 |
| c.2014G>A (p.Glu672Lys) | Missense | <0.1% | Unknown significance; observed in breast cancer |
Mutation functional classification
Loss of Function (LOF)
No well-characterized loss-of-function mutations reported in SRPK2; most variants are missense with uncertain impact.
Gain of Function (GOF)
Overexpression and activating mutations (e.g., p.Arg396Trp) are associated with increased kinase activity and oncogenic splicing.
Dominant Negative (DN)
No dominant-negative mutations described for SRPK2.
View complete mutation data：
Gene Ontology (GO)
Pathways
• Alternative splicing (Reactome: R-HSA-72172)
• SRP-dependent cotranslational protein targeting to membrane (Reactome: R-HSA-1799339)
• Signaling by Receptor Tyrosine Kinases (Reactome: R-HSA-9006934)
Protein Summary
SRPK2 is a 688-amino-acid serine/arginine-rich protein kinase that localizes to both nucleus and cytoplasm. It specifically phosphorylates SR splicing factors (e.g., SRSF1, SRSF2) at serine residues in RS domains, modulating their nuclear import and spliceosome assembly. The protein contains a bipartite nuclear localization signal and a kinase domain. SRPK2 activity is regulated by cell cycle-dependent phosphorylation and interaction with molecular chaperones. Dysregulation of SRPK2 contributes to aberrant splicing in cancer and neurodegeneration.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| SRPK2 Knockout HEK293 Cell Line | EDJ-KQ5844 | Human | 6733 | Details Get a Quote |
| SRPK2 Knockout A-549 Cell Line | EDJ-KQ29300 | Human | 6733 | Details Get a Quote |
| SRPK2 Knockout HCT 116 Cell Line | EDJ-KQ29301 | Human | 6733 | Details Get a Quote |
| SRPK2 Knockout HeLa Cell Line | EDJ-KQ29302 | Human | 6733 | Details Get a Quote |
