RAB25: A Small GTPase Involved in Vesicular Trafficking and Cancer
Comprehensive gene card for RAB25, including expression, mutations, and disease associations.
Gene Information Card
| Symbol | RAB25 |
|---|---|
| Full Name | RAB25, member RAS oncogene family |
| Gene Type | Protein coding |
| Chromosomal Location | 1q22 |
| NCBI Gene ID | 57111 ncbi.nlm.nih.gov/gene/57111 |
| Ensembl ID | ENSG00000132680 |
| UniProt ID | P57735 |
| OMIM ID | 602789 |
| HGNC ID | 18238 |
| Aliases | CATX-8, RAB11C, RAB25L1 |
Description
RAB25 is a member of the RAS oncogene family of small GTPases, specifically belonging to the RAB11 subfamily. It regulates vesicular trafficking, particularly recycling endosomes, and is implicated in cell polarity, proliferation, and migration. Overexpression or loss of RAB25 is associated with various cancers, including ovarian, breast, and lung cancer.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Ovarian cancer | RAB25 overexpression promotes tumor growth and invasion via enhanced recycling of growth factor receptors. | PMID: 15199144, COSMIC |
| Breast cancer | RAB25 amplification and overexpression correlate with poor prognosis and metastasis. | PMID: 20010871, COSMIC |
| Lung adenocarcinoma | RAB25 loss of expression contributes to tumor progression and epithelial-mesenchymal transition. | PMID: 23542377, COSMIC |
| Bladder cancer | RAB25 downregulation is associated with invasive phenotype and reduced survival. | PMID: 19137074, COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Kidney | 12.5 | Medium |
| Liver | 8.3 | Medium |
| Lung | 6.7 | Low |
| Ovary | 15.2 | Medium |
| Breast | 9.1 | Medium |
| Colon | 4.5 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 14.8 | Cervical cancer cell line |
| MCF7 | 11.2 | Breast cancer cell line |
| A549 | 6.5 | Lung cancer cell line |
| OVCAR3 | 18.3 | Ovarian cancer cell line |
| HT-29 | 5.1 | Colon cancer cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.47G>A (p.Arg16His) | Missense | <1% | Unknown functional effect; reported in COSMIC |
| c.124C>T (p.Arg42Cys) | Missense | <1% | Potential loss of function; observed in ovarian cancer |
| c.205G>A (p.Glu69Lys) | Missense | <1% | Gain of function; associated with increased cell proliferation |
| c.337C>T (p.Arg113Trp) | Missense | <1% | Unknown; reported in lung adenocarcinoma |
Mutation functional classification
Loss of Function (LOF)
Loss-of-function mutations (e.g., p.Arg42Cys) impair GTP binding and vesicle recycling, contributing to tumor suppression loss.
Gain of Function (GOF)
Gain-of-function mutations (e.g., p.Glu69Lys) enhance GTPase activity, promoting oncogenic signaling and cell migration.
Dominant Negative (DN)
Dominant-negative mutations (e.g., p.Ser27Asn) interfere with wild-type RAB25 function, disrupting endosomal trafficking.
View complete mutation data：
Gene Ontology (GO)
Pathways
• RAB25 regulates endosomal recycling (Reactome: R-HSA-8856828)
• RAB25 in epithelial cell polarity (KEGG: hsa04530)
• RAB25 in cancer pathways (KEGG: hsa05200)
Protein Summary
RAB25 is a 213-amino-acid small GTPase (UniProt P57735) that cycles between active GTP-bound and inactive GDP-bound states. It localizes to recycling endosomes and the plasma membrane, regulating the trafficking of integrins, growth factor receptors, and cadherins. Its expression is tissue-specific, with highest levels in kidney and ovary. Dysregulation of RAB25 is linked to cancer progression, where it can act as either an oncogene or tumor suppressor depending on context.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| RAB25 Knockout HEK293 Cell Line | EDJ-KQ14977 | Human | 57111 | Details Get a Quote |
| RAB25 Knockout HCT 116 Cell Line | EDJ-KQ45471 | Human | 57111 | Details Get a Quote |
| RAB25 Knockout HeLa Cell Line | EDJ-KQ56804 | Human | 57111 | Details Get a Quote |
| RAB25 Knockout A-549 Cell Line | EDJ-KQ65310 | Human | 57111 | Details Get a Quote |
