PRMT3: Protein Arginine Methyltransferase 3
A type I arginine methyltransferase involved in ribosomal RNA processing and cellular stress responses
Gene Information Card
| Symbol | PRMT3 |
|---|---|
| Full Name | Protein arginine methyltransferase 3 |
| Gene Type | Protein coding |
| Chromosomal Location | 11p15.1 |
| NCBI Gene ID | 10196 ncbi.nlm.nih.gov/gene/10196 |
| Ensembl ID | ENSG00000185238 |
| UniProt ID | O60678 |
| OMIM ID | 603191 |
| HGNC ID | 9349 |
| Aliases | HRMT1L3, MGC117283 |
Description
PRMT3 encodes a type I protein arginine methyltransferase that catalyzes the formation of asymmetric dimethylarginine (ADMA) on target proteins. The enzyme is predominantly cytoplasmic and plays a critical role in ribosomal RNA processing by methylating the 40S ribosomal protein S2 (RPS2). PRMT3 is also implicated in cellular stress responses, mRNA splicing, and signal transduction. Its activity is regulated by homodimerization and interaction with the 40S ribosomal subunit.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (multiple types) | PRMT3 overexpression promotes cell proliferation and migration via methylation of RPS2 and other substrates; altered ADMA levels affect signaling pathways. | PMID: 25605274, 30356049 |
| Neurodevelopmental disorders | PRMT3 variants (e.g., p.Arg68Cys) impair ribosomal RNA processing and are associated with intellectual disability and microcephaly. | PMID: 31036916 |
| Cardiovascular disease | PRMT3-mediated ADMA production contributes to endothelial dysfunction and atherosclerosis. | PMID: 23934898 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Brain | 8.3 | Medium |
| Heart | 6.1 | Low |
| Liver | 4.7 | Low |
| Kidney | 5.9 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 15.2 | High expression |
| HeLa | 10.8 | Medium expression |
| K562 | 7.4 | Low expression |
| HepG2 | 6.1 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.202C>T (p.Arg68Cys) | Missense | Rare | Loss of function; reduced methyltransferase activity and impaired ribosome assembly |
| c.1045G>A (p.Glu349Lys) | Missense | Rare | Gain of function; increased ADMA production in vitro |
| c.1234_1235insA (p.Thr412Asnfs*5) | Frameshift | Very rare | Loss of function; truncated protein |
Mutation functional classification
Loss of Function (LOF)
p.Arg68Cys and frameshift variants reduce or abolish methyltransferase activity, leading to defective rRNA processing and impaired cell growth.
Gain of Function (GOF)
p.Glu349Lys increases catalytic activity, potentially promoting oncogenic signaling.
Dominant Negative (DN)
No dominant-negative mutations reported in PRMT3.
View complete mutation data：
Gene Ontology (GO)
| • RNA binding (GO:0003723) | • nucleus (GO:0005634) |
| • cytoplasm (GO:0005737) | • protein methylation (GO:0006479) |
| • histone arginine N-methyltransferase activity (GO:0008469) | • protein-arginine N-methyltransferase activity (GO:0016274) |
| • methylation (GO:0032259) | • ribosomal small subunit biogenesis (GO:0042274) |
Pathways
• Ribosome biogenesis (R-HSA-72689)
• Arginine methylation (R-HSA-8878171)
• mRNA splicing (R-HSA-72163)
Protein Summary
PRMT3 is a 531-amino acid type I arginine methyltransferase that asymmetrically dimethylates arginine residues on target proteins. It contains a conserved methyltransferase domain and a zinc finger motif required for substrate recognition. The enzyme forms homodimers and interacts with the 40S ribosomal subunit via RPS2. PRMT3 is essential for proper ribosome assembly and translation efficiency. Dysregulation of PRMT3 expression or activity is linked to cancer, neurodevelopmental disorders, and cardiovascular disease.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PRMT3 Knockout HEK293 Cell Line | EDJ-KQ1100 | Human | 10196 | Details Get a Quote |
| PRMT3 Knockout A-549 Cell Line | EDJ-KQ20276 | Human | 10196 | Details Get a Quote |
| PRMT3 Knockout HCT 116 Cell Line | EDJ-KQ20277 | Human | 10196 | Details Get a Quote |
| PRMT3 Knockout HeLa Cell Line | EDJ-KQ20278 | Human | 10196 | Details Get a Quote |
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