PRKAR2A
Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha
Gene Information Card
| Symbol | PRKAR2A |
|---|---|
| Full Name | Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha |
| Gene Type | protein-coding |
| Chromosomal Location | 3p21.31 |
| NCBI Gene ID | 5576 ncbi.nlm.nih.gov/gene/5576 |
| Ensembl ID | ENSG00000114346 |
| UniProt ID | P13861 |
| OMIM ID | 176910 |
| HGNC ID | 9392 |
| Aliases | PKR2, PRKAR2, RII-alpha |
Description
PRKAR2A encodes the regulatory subunit type II-alpha of cAMP-dependent protein kinase A (PKA). This subunit binds cAMP and regulates PKA catalytic activity, playing a key role in signal transduction pathways controlling metabolism, gene expression, and cell proliferation. Mutations in PRKAR2A are linked to Carney complex and other neoplasia syndromes.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Carney complex | Loss of function leads to dysregulated PKA activity and tumorigenesis | OMIM #160980; ClinVar |
| Pigmented nodular adrenocortical disease | Impaired cAMP signaling in adrenal cortex | OMIM #610489; NCBI |
| Thyroid adenoma | Altered PKA regulation in thyroid follicular cells | COSMIC; literature |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Heart | 9.8 | Medium |
| Adrenal gland | 15.2 | High |
| Thyroid | 11.0 | Medium |
| Testis | 8.3 | Medium |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 14.1 | High expression |
| HeLa | 10.5 | Moderate expression |
| K562 | 7.2 | Low expression |
| A549 | 9.0 | Moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.892C>T (p.Arg298*) | Nonsense | <0.1% | Loss of function; associated with Carney complex |
| c.1A>G (p.Met1?) | Start loss | <0.1% | Loss of function; reported in adrenal tumors |
| c.1003G>A (p.Gly335Arg) | Missense | <0.1% | Dominant negative effect on PKA regulation |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations leading to truncated protein or nonsense-mediated decay.
Gain of Function (GOF)
Not commonly reported; some missense variants may increase cAMP binding affinity.
Dominant Negative (DN)
Missense mutations (e.g., p.Gly335Arg) that impair regulatory subunit function and cause constitutive PKA activity.
View complete mutation data：
Gene Ontology (GO)
| • cAMP-dependent protein kinase regulator activity | • protein kinase A binding |
| • cAMP binding | • signal transduction |
| • regulation of protein phosphorylation |
Pathways
• cAMP/PKA signaling pathway
• GPCR downstream signaling
• Regulation of lipolysis in adipocytes
Protein Summary
The PRKAR2A protein (RII-alpha) is a regulatory subunit of PKA type II. It contains two cAMP-binding domains and an N-terminal dimerization/docking domain. Upon cAMP binding, it releases the catalytic subunits to phosphorylate downstream targets. It is highly expressed in brain, heart, and endocrine tissues.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PRKAR2A Knockout HEK293 Cell Line | EDJ-KQ3483 | Human | 5576 | Details Get a Quote |
| PRKAR2A Knockout A-549 Cell Line | EDJ-KQ25257 | Human | 5576 | Details Get a Quote |
| PRKAR2A Knockout HCT 116 Cell Line | EDJ-KQ25258 | Human | 5576 | Details Get a Quote |
| PRKAR2A Knockout HeLa Cell Line | EDJ-KQ25259 | Human | 5576 | Details Get a Quote |
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