PRAG1: PEAK1 Related, Kinase-Activating Pseudokinase 1
A pseudokinase involved in cell migration and cancer signaling
Gene Information Card
| Symbol | PRAG1 |
|---|---|
| Full Name | PEAK1 Related, Kinase-Activating Pseudokinase 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 8p11.23 |
| NCBI Gene ID | 157285 ncbi.nlm.nih.gov/gene/157285 |
| Ensembl ID | ENSG00000164741 |
| UniProt ID | Q86YV5 |
| OMIM ID | 617362 |
| HGNC ID | 28967 |
| Aliases | C8orf34, FLJ14213, PRAGMIN |
Description
PRAG1 (PEAK1 Related, Kinase-Activating Pseudokinase 1) is a protein-coding gene located on chromosome 8p11.23. It encodes a pseudokinase that belongs to the PEAK family and is involved in regulating cell migration, cytoskeletal dynamics, and signaling pathways relevant to cancer progression. PRAG1 interacts with and activates the kinase PEAK1, contributing to focal adhesion turnover and cell motility.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (general) | PRAG1 overexpression promotes cell migration and invasion via PEAK1 activation | PMID: 25944712 |
| Breast cancer | PRAG1 upregulation correlates with poor prognosis and metastatic potential | PMID: 25944712 |
| Colorectal cancer | PRAG1 expression linked to tumor progression and metastasis | PMID: 25944712 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 3.2 | Low |
| Heart | 1.8 | Low |
| Kidney | 4.5 | Medium |
| Liver | 2.1 | Low |
| Lung | 5.0 | Medium |
| Pancreas | 1.5 | Low |
| Placenta | 6.8 | Medium |
| Skeletal muscle | 0.9 | Not detected |
| Small intestine | 3.9 | Low |
| Spleen | 2.3 | Low |
| Testis | 7.2 | Medium |
| Thymus | 1.1 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 4.8 | Embryonic kidney cells |
| HeLa | 6.2 | Cervical cancer cells |
| MCF7 | 5.5 | Breast cancer cells |
| HCT116 | 7.0 | Colorectal cancer cells |
| A549 | 5.1 | Lung cancer cells |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1045C>T (p.Arg349Trp) | Missense | <0.01% | Unknown functional effect |
| c.1573G>A (p.Val525Ile) | Missense | <0.01% | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in PRAG1.
Gain of Function (GOF)
Overexpression of wild-type PRAG1 is associated with gain-of-function in cell migration and invasion.
Dominant Negative (DN)
No dominant-negative mutations described.
View complete mutation data：
Gene Ontology (GO)
| • protein binding (GO:0005515) | • protein kinase activity (GO:0004672) |
| • actin cytoskeleton organization (GO:0030036) | • cell migration (GO:0016477) |
Pathways
• PEAK1 signaling pathway
• Focal adhesion pathway
Protein Summary
PRAG1 encodes a 1,284-amino acid pseudokinase protein with a conserved kinase-like domain but lacks catalytic activity. It functions as a scaffold that binds and activates the tyrosine kinase PEAK1, promoting downstream signaling through SRC and FAK. PRAG1 localizes to focal adhesions and regulates cell migration, invasion, and cytoskeletal remodeling. Its overexpression in various cancers suggests a role in tumor progression.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| PRAG1 Knockout HEK293 Cell Line | EDJ-KQ14868 | Human | 157285 | Details Get a Quote |
| PRAG1 Knockout A-549 Cell Line | EDJ-KQ45326 | Human | 157285 | Details Get a Quote |
| PRAG1 Knockout HCT 116 Cell Line | EDJ-KQ45327 | Human | 157285 | Details Get a Quote |
| PRAG1 Knockout HeLa Cell Line | EDJ-KQ45328 | Human | 157285 | Details Get a Quote |
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