NCK1: A Key Adaptor Protein in Signal Transduction
Comprehensive gene information for NCK1, including function, expression, and disease associations.
Gene Information Card
| Symbol | NCK1 |
|---|---|
| Full Name | NCK adaptor protein 1 |
| Gene Type | protein-coding |
| Chromosomal Location | 3q22.3 |
| NCBI Gene ID | 4690 ncbi.nlm.nih.gov/gene/4690 |
| Ensembl ID | ENSG00000114771 |
| UniProt ID | P16333 |
| OMIM ID | 600508 |
| HGNC ID | 7664 |
| Aliases | NCK, NCKalpha, SH2/SH3 adaptor protein NCK-alpha |
Description
NCK1 encodes a member of the NCK family of adaptor proteins, which contain one SH2 domain and three SH3 domains. This protein functions as a signal transducer, linking receptor tyrosine kinases to downstream signaling pathways, including those involved in cell growth, migration, and cytoskeletal reorganization. It is ubiquitously expressed and plays a role in various cellular processes.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | NCK1 overexpression may promote cell migration and invasion via altered signaling | PMID: 23454750 |
| Prostate cancer | NCK1 amplification or overexpression linked to tumor progression | PMID: 25691885 |
| Renal cell carcinoma | NCK1 upregulation associated with poor prognosis | PMID: 27323851 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Lung | 8.3 | Low |
| Liver | 6.1 | Low |
| Kidney | 15.2 | Medium |
| Testis | 20.4 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 18.7 | High expression |
| HeLa | 14.2 | Medium expression |
| MCF7 | 22.1 | High expression |
| A549 | 9.8 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.101C>T (p.Thr34Met) | Missense | <0.1% | Unknown functional impact |
| c.457G>A (p.Glu153Lys) | Missense | <0.1% | Reported in COSMIC; potential gain-of-function |
| c.784_786del (p.Lys262del) | In-frame deletion | <0.1% | Loss of SH3 domain function |
Mutation functional classification
Loss of Function (LOF)
Deletions in SH3 domains (e.g., p.Lys262del) impair protein-protein interactions.
Gain of Function (GOF)
Missense mutations like p.Glu153Lys may enhance signaling in cancer contexts.
Dominant Negative (DN)
Not well characterized; some SH2 domain mutants may act dominant-negative.
View complete mutation data：
Gene Ontology (GO)
| • SH3/SH2 adaptor activity (GO:0005070) | • signal transduction (GO:0007165) |
| • actin cytoskeleton organization (GO:0030036) | • neurotrophin TRK receptor signaling pathway (GO:0048011) |
| • cytoplasm (GO:0005737) |
Pathways
• EGFR signaling pathway (Reactome: R-HSA-177929)
• VEGF signaling pathway (Reactome: R-HSA-194138)
• PDGF receptor signaling (Reactome: R-HSA-186797)
Protein Summary
NCK1 is a 377-amino acid adaptor protein with one SH2 and three SH3 domains. It binds phosphorylated tyrosine residues on activated receptors via its SH2 domain and recruits downstream effectors (e.g., PAK, WASP) via SH3 domains, regulating cytoskeletal dynamics and cell migration. It is widely expressed and implicated in cancer progression.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| NCK1 Knockout HEK293 Cell Line | EDJ-KQ3233 | Human | 4690 | Details Get a Quote |
| NCK1 Knockout A-549 Cell Line | EDJ-KQ24742 | Human | 4690 | Details Get a Quote |
| NCK1 Knockout HCT 116 Cell Line | EDJ-KQ24743 | Human | 4690 | Details Get a Quote |
| NCK1 Knockout HeLa Cell Line | EDJ-KQ24744 | Human | 4690 | Details Get a Quote |
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