MOB1A
MOB kinase activator 1A
Gene Information Card
| Symbol | MOB1A |
|---|---|
| Full Name | MOB kinase activator 1A |
| Gene Type | protein-coding |
| Chromosomal Location | 2p13.1 |
| NCBI Gene ID | 55233 ncbi.nlm.nih.gov/gene/55233 |
| Ensembl ID | ENSG00000115942 |
| UniProt ID | Q9H8S9 |
| OMIM ID | 609282 |
| HGNC ID | 29802 |
| Aliases | MOB1, MOBKL1B, MATS1, MOB4B |
Description
MOB1A encodes a member of the MOB family of proteins that function as co-activators of the Hippo signaling pathway. The protein interacts with and activates serine/threonine kinases STK3 (MST2) and STK4 (MST1), leading to phosphorylation and activation of LATS1/2 kinases, which in turn phosphorylate and inhibit YAP1 and WWTR1 (TAZ) transcriptional co-activators. This pathway regulates organ size, cell proliferation, and apoptosis. MOB1A is ubiquitously expressed and plays a critical role in tumor suppression.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | Loss of MOB1A expression leads to YAP/TAZ nuclear translocation and uncontrolled proliferation | PMID: 25652263 |
| Colorectal cancer | Reduced MOB1A levels correlate with poor prognosis and activation of Hippo pathway targets | PMID: 29162811 |
| Breast cancer | MOB1A downregulation promotes metastasis via YAP1 activation | PMID: 30356049 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 12.5 | Medium |
| Kidney | 10.8 | Medium |
| Heart | 8.2 | Low |
| Brain | 6.1 | Low |
| Lung | 9.4 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 14.3 | Hepatocellular carcinoma cell line |
| MCF7 | 11.0 | Breast cancer cell line |
| A549 | 9.8 | Lung cancer cell line |
| HEK293 | 7.5 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.205C>T (p.Arg69Trp) | Missense | <0.1% | Impaired binding to STK3/STK4, reduced Hippo signaling |
| c.346G>A (p.Glu116Lys) | Missense | <0.1% | Loss of kinase activation function |
| c.1A>G (p.Met1Val) | Start loss | Rare | Complete loss of protein expression |
Mutation functional classification
Loss of Function (LOF)
Missense mutations (e.g., p.Arg69Trp) disrupt interaction with MST kinases, impairing LATS1/2 activation and leading to YAP/TAZ nuclear accumulation.
Gain of Function (GOF)
No gain-of-function mutations reported in MOB1A.
Dominant Negative (DN)
No dominant-negative mutations described for MOB1A.
View complete mutation data：
Gene Ontology (GO)
| • protein kinase activity (GO:0004672) | • protein binding (GO:0005515) |
| • hippo signaling (GO:0035329) | • identical protein binding (GO:0042802) |
| • molecular adaptor activity (GO:0060090) |
Pathways
• Hippo signaling pathway (KEGG: hsa04390)
• Regulation of organ size (Reactome: R-HSA-2028269)
• YAP/TAZ regulation (Reactome: R-HSA-8939211)
Protein Summary
MOB1A is a 216-amino-acid protein (25 kDa) that contains a conserved MOB1/phocein domain. It functions as a scaffold and activator of the Hippo kinase cascade. The protein binds to MST1/2 via its N-terminal region and to LATS1/2 via its C-terminal region, facilitating phosphorylation events. MOB1A is predominantly cytoplasmic but can shuttle to the nucleus upon pathway activation. Post-translational modifications include phosphorylation at Ser146 by MST1/2, which enhances its activity.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MOB1A Knockout HEK293 Cell Line | EDJ-KQ1376 | Human | 55233 | Details Get a Quote |
| MOB1A Knockout A-549 Cell Line | EDJ-KQ20877 | Human | 55233 | Details Get a Quote |
| MOB1A Knockout HCT 116 Cell Line | EDJ-KQ20878 | Human | 55233 | Details Get a Quote |
| MOB1A Knockout HeLa Cell Line | EDJ-KQ20879 | Human | 55233 | Details Get a Quote |
