MISP (Mitotic Spindle Positioning) Gene
Comprehensive gene card for MISP, a regulator of mitotic spindle orientation and cell polarity.
Gene Information Card
| Symbol | MISP |
|---|---|
| Full Name | Mitotic Spindle Positioning |
| Gene Type | Protein coding |
| Chromosomal Location | 19p13.3 |
| NCBI Gene ID | 126353 ncbi.nlm.nih.gov/gene/126353 |
| Ensembl ID | ENSG00000167552 |
| UniProt ID | Q8TAC9 |
| OMIM ID | 611488 |
| HGNC ID | 27000 |
| Aliases | C19orf21, MISP1, FLJ11273 |
Description
MISP (Mitotic Spindle Positioning) is a protein-coding gene located on chromosome 19p13.3. It encodes a protein involved in the regulation of mitotic spindle orientation, cell polarity, and asymmetric cell division. MISP interacts with the actin cytoskeleton and the microtubule network to ensure proper spindle positioning during mitosis. Dysregulation of MISP has been implicated in cancer progression and metastasis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | MISP overexpression promotes cell migration and invasion through altered spindle orientation and polarity defects. | PMID: 23431171 |
| Colorectal cancer | MISP upregulation correlates with poor prognosis and increased metastatic potential. | PMID: 25944712 |
| Lung cancer | MISP expression is elevated in non-small cell lung cancer and associated with tumor aggressiveness. | PMID: 27323850 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Testis | 12.5 | Medium |
| Bone marrow | 8.2 | Low |
| Lymph node | 6.7 | Low |
| Brain | 4.1 | Low |
| Breast | 3.9 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 15.3 | Cervical cancer cell line |
| MCF7 | 11.8 | Breast cancer cell line |
| A549 | 9.4 | Lung cancer cell line |
| HCT116 | 8.7 | Colorectal cancer cell line |
| HEK293 | 6.2 | Embryonic kidney cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1015C>T (p.Arg339Trp) | Missense | <0.01% | Unknown functional effect |
| c.1246G>A (p.Glu416Lys) | Missense | <0.01% | Unknown functional effect |
| c.1489_1491del (p.Glu497del) | In-frame deletion | <0.01% | Unknown functional effect |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in MISP.
Gain of Function (GOF)
Overexpression of wild-type MISP is associated with gain-of-function effects in cancer, but specific gain-of-function mutations are not characterized.
Dominant Negative (DN)
No dominant-negative mutations have been described for MISP.
View complete mutation data：
Gene Ontology (GO)
Pathways
• REACT:14797 (Mitotic Spindle Positioning)
• REACT:172623 (Cell Cycle
• Mitotic)
Protein Summary
The MISP protein (UniProt Q8TAC9) is a 647-amino acid protein that localizes to the centrosome and the cell cortex. It contains an N-terminal calponin homology (CH) domain that binds actin, and a C-terminal region that interacts with microtubules. MISP regulates the positioning of the mitotic spindle by linking the astral microtubules to the cortical actin cytoskeleton. It is essential for asymmetric cell division and proper orientation of the mitotic spindle, which are critical for tissue morphogenesis and stem cell maintenance.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MISP Knockout HEK293 Cell Line | EDJ-KQ3156 | Human | 126353 | Details Get a Quote |
| MISP3 Knockout HEK293 Cell Line | EDJ-KQ7414 | Human | 113230 | Details Get a Quote |
| MISP Knockout A-549 Cell Line | EDJ-KQ24559 | Human | 126353 | Details Get a Quote |
| MISP Knockout HCT 116 Cell Line | EDJ-KQ24560 | Human | 126353 | Details Get a Quote |
| MISP Knockout HeLa Cell Line | EDJ-KQ24561 | Human | 126353 | Details Get a Quote |
| MISP3 Knockout A-549 Cell Line | EDJ-KQ32591 | Human | 113230 | Details Get a Quote |
| MISP3 Knockout HCT 116 Cell Line | EDJ-KQ32592 | Human | 113230 | Details Get a Quote |
| MISP3 Knockout HeLa Cell Line | EDJ-KQ32593 | Human | 113230 | Details Get a Quote |
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