MICAL3: Microtubule Associated Monooxygenase, Calponin And LIM Domain Containing 3
A key regulator of vesicle trafficking, cytoskeletal dynamics, and neuronal development
Gene Information Card
| Symbol | MICAL3 |
|---|---|
| Full Name | Microtubule Associated Monooxygenase, Calponin And LIM Domain Containing 3 |
| Gene Type | protein-coding |
| Chromosomal Location | 22q11.21 |
| NCBI Gene ID | 57553 ncbi.nlm.nih.gov/gene/57553 |
| Ensembl ID | ENSG00000100219 |
| UniProt ID | Q8R4Y1 |
| OMIM ID | 608382 |
| HGNC ID | 24694 |
| Aliases | KIAA0819, MICAL-3, FLJ10154 |
Description
MICAL3 encodes a member of the MICAL family of proteins, which are flavoprotein monooxygenases that regulate actin cytoskeleton dynamics and vesicle trafficking. The protein contains a calponin homology (CH) domain, a LIM domain, and a monooxygenase domain. MICAL3 is involved in neuronal development, cell migration, and intracellular transport, and its dysregulation has been implicated in cancer and neurological disorders.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various types) | Altered MICAL3 expression may affect cell migration and invasion via actin remodeling | COSMIC, literature |
| Neurodevelopmental disorders | Mutations in MICAL3 may disrupt neuronal vesicle trafficking and axon guidance | OMIM, literature |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Lung | 8.3 | Low |
| Liver | 6.1 | Low |
| Kidney | 9.7 | Low |
| Testis | 15.2 | Medium |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 10.1 | Cervical cancer cell line |
| HEK293 | 8.5 | Embryonic kidney cell line |
| SH-SY5Y | 14.3 | Neuroblastoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T (p.Arg412*) | Nonsense | <0.1% | Loss of function |
| c.567G>A (p.Trp189*) | Nonsense | <0.1% | Loss of function |
| c.890A>G (p.Tyr297Cys) | Missense | <0.1% | Unknown |
Mutation functional classification
Loss of Function (LOF)
Nonsense mutations (e.g., p.Arg412*, p.Trp189*) lead to truncated protein and loss of monooxygenase activity.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
No confirmed dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • actin filament organization | • vesicle-mediated transport |
| • oxidation-reduction process | • calponin homology domain binding |
| • FAD binding | • LIM domain binding |
Pathways
• Semaphorin signaling
• Actin cytoskeleton regulation
• Vesicle trafficking
Protein Summary
MICAL3 is a 2001-amino acid flavoprotein monooxygenase that localizes to the cytoplasm and associates with microtubules. It catalyzes the oxidation of actin methionine residues, leading to actin filament disassembly, and interacts with Rab GTPases to regulate vesicle docking and fusion. The protein is highly expressed in the brain and testis, and its activity is critical for neuronal growth cone collapse and cell migration.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MICAL3 Knockout HEK293 Cell Line | EDJ-KQ14258 | Human | 57553 | Details Get a Quote |
| MICAL3 Knockout A-549 Cell Line | EDJ-KQ44263 | Human | 57553 | Details Get a Quote |
| MICAL3 Knockout HCT 116 Cell Line | EDJ-KQ44264 | Human | 57553 | Details Get a Quote |
| MICAL3 Knockout HeLa Cell Line | EDJ-KQ44265 | Human | 57553 | Details Get a Quote |
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