MARK3 Gene
Microtubule Affinity Regulating Kinase 3
Gene Information Card
| Symbol | MARK3 |
|---|---|
| Full Name | Microtubule Affinity Regulating Kinase 3 |
| Gene Type | protein-coding |
| Chromosomal Location | 14q32.33 |
| NCBI Gene ID | 4140 ncbi.nlm.nih.gov/gene/4140 |
| Ensembl ID | ENSG00000100811 |
| UniProt ID | P27448 |
| OMIM ID | 602678 |
| HGNC ID | 6897 |
| Aliases | C-TAK1, PAR1A, KP78, MARK3_HUMAN |
Description
MARK3 is a member of the AMPK-related kinase family that phosphorylates microtubule-associated proteins (MAPs) such as MAPT/tau, regulating microtubule stability and cell polarity. It also acts as a scaffold in signaling pathways, including the Hippo pathway, and is involved in cell cycle control and apoptosis.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Cancer (various) | MARK3 dysregulation alters cell polarity and proliferation; loss of function promotes tumorigenesis | COSMIC, ClinVar |
| Alzheimer Disease | MARK3 phosphorylates tau at sites associated with neurofibrillary tangles | UniProt, NCBI |
| Hepatocellular Carcinoma | MARK3 downregulation correlates with poor prognosis and metastasis | NCBI, COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 15.2 | Medium |
| Liver | 10.8 | Medium |
| Kidney | 9.5 | Low |
| Heart | 7.3 | Low |
| Testis | 20.1 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 18.5 | High expression |
| HeLa | 12.3 | Medium expression |
| HepG2 | 9.8 | Low expression |
| SH-SY5Y | 22.1 | High expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1072C>T (p.Arg358Trp) | Missense | <0.01% | Unknown; predicted damaging |
| c.1543G>A (p.Glu515Lys) | Missense | <0.01% | Unknown; predicted benign |
| c.1861_1863del (p.Lys621del) | In-frame deletion | <0.01% | Unknown; may affect kinase activity |
Mutation functional classification
Loss of Function (LOF)
Reduced kinase activity or protein stability; associated with cancer progression.
Gain of Function (GOF)
Not well documented; potential hyperactivation in some cancers.
Dominant Negative (DN)
Not reported for MARK3.
View complete mutation data：
Gene Ontology (GO)
| • protein serine/threonine kinase activity | • ATP binding |
| • microtubule binding | • cell cycle |
| • cell polarity | • Hippo signaling |
Pathways
• Hippo signaling pathway
• Tau protein phosphorylation
• AMPK signaling
Protein Summary
MARK3 is a 753-amino acid serine/threonine kinase with an N-terminal kinase domain, a ubiquitin-associated domain, and a C-terminal KA1 domain. It phosphorylates tau and other MAPs, regulating microtubule dynamics and cell polarity. It also interacts with STRAD and MO25 to form a complex that activates LKB1, linking energy sensing to cell growth.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| MARK3 Knockout HEK293 Cell Line | EDJ-KQ5179 | Human | 4140 | Details Get a Quote |
| MARK3 Knockout A-549 Cell Line | EDJ-KQ28166 | Human | 4140 | Details Get a Quote |
| MARK3 Knockout HCT 116 Cell Line | EDJ-KQ28167 | Human | 4140 | Details Get a Quote |
| MARK3 Knockout HeLa Cell Line | EDJ-KQ28168 | Human | 4140 | Details Get a Quote |
| MARK3 Knockout HAP1 Cell Line | EDC07783 | Human | 4140 | Details Get a Quote |
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