GPR35: G Protein-Coupled Receptor 35
A comprehensive biomedical overview of GPR35, including gene characteristics, expression, mutations, and disease associations.
Gene Information Card
| Symbol | GPR35 |
|---|---|
| Full Name | G protein-coupled receptor 35 |
| Gene Type | protein-coding |
| Chromosomal Location | 2q37.3 |
| NCBI Gene ID | 2859 ncbi.nlm.nih.gov/gene/2859 |
| Ensembl ID | ENSG00000178623 |
| UniProt ID | Q9HC97 |
| OMIM ID | 602668 |
| HGNC ID | 4492 |
| Aliases | KPG_001, GPR35a, GPR35b |
Description
GPR35 encodes a member of the G protein-coupled receptor family, specifically a class A orphan receptor. The protein is involved in various physiological processes, including immune response, gastrointestinal function, and cardiovascular regulation. It is activated by ligands such as kynurenic acid and zaprinast, and signals primarily through Gα13 and Gαi/o pathways.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Inflammatory Bowel Disease | GPR35 variants may alter receptor signaling, affecting immune cell recruitment and gut inflammation. | GWAS studies (PMID: 26192919) |
| Hypertension | GPR35 expression in vascular smooth muscle cells modulates blood pressure via Gα13 signaling. | Animal models (PMID: 29127202) |
| Cancer (colorectal) | GPR35 overexpression promotes tumor growth and metastasis through MAPK/ERK pathway activation. | Expression analysis (COSMIC, PMID: 25691885) |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Small Intestine | 12.5 | Medium |
| Colon | 10.8 | Medium |
| Stomach | 8.2 | Low |
| Spleen | 6.4 | Low |
| Lung | 4.1 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK293 | 15.2 | High expression in recombinant systems |
| HT-29 | 9.8 | Colorectal adenocarcinoma cell line |
| THP-1 | 7.5 | Monocytic leukemia cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.100C>T (p.Arg34Trp) | Missense | 0.01% (gnomAD) | Reduced ligand binding affinity |
| c.389G>A (p.Arg130His) | Missense | 0.005% (gnomAD) | Altered G protein coupling |
| c.715C>T (p.Arg239Trp) | Missense | 0.02% (gnomAD) | Loss of function in signaling assays |
Mutation functional classification
Loss of Function (LOF)
p.Arg239Trp reduces receptor activation and downstream signaling.
Gain of Function (GOF)
No confirmed gain-of-function mutations reported.
Dominant Negative (DN)
Not described for GPR35.
View complete mutation data：
Gene Ontology (GO)
| • G protein-coupled receptor activity (GO:0004930) | • G protein-coupled receptor signaling pathway (GO:0007186) |
| • plasma membrane (GO:0005886) | • signal transduction (GO:0007165) |
Pathways
• G alpha (13) signaling events
• GPCR downstream signaling
• Kynurenic acid signaling
Protein Summary
GPR35 is a 309-amino acid transmembrane receptor with seven helical domains. It is expressed in immune and gastrointestinal tissues and mediates cellular responses to kynurenic acid and other ligands. The receptor couples to Gα13 and Gαi/o, regulating RhoA and ERK pathways. Structural studies indicate a conserved ligand-binding pocket typical of class A GPCRs.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| GPR35 Knockout HEK293 Cell Line | EDJ-KQ4771 | Human | 2859 | Details Get a Quote |
| GPR35 Knockout A-549 Cell Line | EDJ-KQ27526 | Human | 2859 | Details Get a Quote |
| GPR35 Knockout HCT 116 Cell Line | EDJ-KQ27527 | Human | 2859 | Details Get a Quote |
| GPR35 Knockout HeLa Cell Line | EDJ-KQ53409 | Human | 2859 | Details Get a Quote |
