EPHB2
EPH Receptor B2: A Key Regulator in Cell Positioning, Migration, and Cancer Progression
Gene Information Card
| Symbol | EPHB2 |
|---|---|
| Full Name | EPH Receptor B2 |
| Gene Type | protein-coding |
| Chromosomal Location | 1p36.12 |
| NCBI Gene ID | 2048 ncbi.nlm.nih.gov/gene/2048 |
| Ensembl ID | ENSG00000133216 |
| UniProt ID | P29323 |
| OMIM ID | 600997 |
| HGNC ID | 3393 |
| Aliases | CAPB, DRT, EPHT3, ERK, Hek5, PCBC, Tyro5 |
Description
EPHB2 (EPH receptor B2) is a member of the Eph receptor tyrosine kinase family, which mediates bidirectional signaling upon binding to ephrin-B ligands. It plays critical roles in cell positioning, migration, axon guidance, and angiogenesis. EPHB2 is frequently altered in various cancers, including colorectal, prostate, and breast cancers, where it can act as either a tumor suppressor or oncogene depending on the cellular context.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal Cancer | Loss of EPHB2 expression disrupts intestinal epithelial cell positioning and promotes tumor progression. | ClinVar, COSMIC |
| Prostate Cancer | EPHB2 mutations and reduced expression are associated with aggressive disease and metastasis. | COSMIC, NCBI Gene |
| Breast Cancer | EPHB2 overexpression correlates with poor prognosis and invasive phenotype. | NCBI Gene, COSMIC |
| Gastric Cancer | EPHB2 downregulation is linked to tumor invasion and lymph node metastasis. | NCBI Gene |
| Lung Cancer | EPHB2 alterations contribute to tumor growth and resistance to therapy. | COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Colon | 8.3 | Medium |
| Prostate | 7.1 | Medium |
| Breast | 6.4 | Low |
| Lung | 5.2 | Low |
| Liver | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HEK 293 | 15.2 | High expression |
| HeLa | 10.1 | Moderate expression |
| MCF7 | 7.8 | Moderate expression |
| PC3 | 6.5 | Low expression |
| A549 | 4.3 | Low expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| p.Gly787Arg | Missense | <1% | Alters kinase domain; potential loss of function |
| p.Arg524* | Nonsense | <1% | Truncation; loss of function |
| p.Val105Ile | Missense | <1% | Unknown significance |
| p.Pro707Leu | Missense | <1% | Unknown significance |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations leading to truncated protein or reduced kinase activity.
Gain of Function (GOF)
Missense mutations in the kinase domain that enhance signaling have been reported in some cancers.
Dominant Negative (DN)
Truncated receptors lacking the kinase domain can interfere with wild-type EPHB2 signaling.
View complete mutation data：
Gene Ontology (GO)
| • receptor tyrosine kinase activity | • transmembrane signaling receptor activity |
| • protein binding | • ATP binding |
| • axon guidance | • cell migration |
| • angiogenesis | • cell adhesion |
| • ephrin receptor signaling pathway |
Pathways
• Ephrin B-EPHB forward signaling
• Ephrin B reverse signaling
• Axon guidance
• Signaling by Receptor Tyrosine Kinases
• EPH-ephrin mediated repulsion of cells
Protein Summary
EPHB2 is a single-pass type I transmembrane receptor with an N-terminal ephrin-binding domain, a cysteine-rich region, two fibronectin type III repeats, a transmembrane segment, and a cytoplasmic tyrosine kinase domain. Upon binding ephrin-B ligands, it undergoes autophosphorylation and activates downstream signaling pathways, including MAPK and PI3K/AKT, regulating cytoskeletal dynamics and cell adhesion. EPHB2 also mediates reverse signaling through its ligand ephrin-B.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| EPHB2 Knockout HEK293 Cell Line | EDJ-KQ2329 | Human | 2048 | Details Get a Quote |
| EPHB2 Knockout HCT 116 Cell Line | EDJ-KQ21402 | Human | 2048 | Details Get a Quote |
| EPHB2 Knockout A-549 Cell Line | EDJ-KQ22725 | Human | 2048 | Details Get a Quote |
| EPHB2 Knockout HeLa Cell Line | EDJ-KQ22727 | Human | 2048 | Details Get a Quote |
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