CRACD (Capping Protein Inhibiting Regulator of Actin Dynamics)
Key regulator of actin capping and cytoskeletal dynamics
Gene Information Card
| Symbol | CRACD |
|---|---|
| Full Name | Capping Protein Inhibiting Regulator of Actin Dynamics |
| Gene Type | Protein coding |
| Chromosomal Location | 4q35.1 |
| NCBI Gene ID | 440275 ncbi.nlm.nih.gov/gene/440275 |
| Ensembl ID | ENSG00000138698 |
| UniProt ID | Q5T0Y3 |
| OMIM ID | 617749 |
| HGNC ID | 28323 |
| Aliases | CRACDL, KIAA1211, MGC138290 |
Description
CRACD (Capping Protein Inhibiting Regulator of Actin Dynamics) encodes a protein that negatively regulates actin capping, thereby promoting actin filament elongation and cytoskeletal remodeling. It is involved in cell migration, adhesion, and morphogenesis. The gene is located on chromosome 4q35.1 and is expressed in multiple tissues.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Colorectal cancer | Altered expression may affect actin dynamics and cell motility | COSMIC, NCBI Gene |
| Breast cancer | Potential role in cytoskeletal regulation and metastasis | COSMIC |
| Lung cancer | Mutations and expression changes observed | COSMIC |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Heart | 8.3 | Low |
| Liver | 6.1 | Low |
| Lung | 15.2 | Medium |
| Kidney | 9.8 | Low |
| Testis | 22.4 | High |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 18.5 | Cervical cancer cell line |
| A549 | 14.2 | Lung cancer cell line |
| MCF7 | 11.3 | Breast cancer cell line |
| HEK293 | 9.7 | Embryonic kidney cells |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1234C>T | Missense | 0.5% | p.Arg412Cys; potential loss of function |
| c.567delA | Frameshift | 0.1% | p.Lys189fs; truncation |
| c.890G>A | Nonsense | 0.2% | p.Trp297*; premature stop |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations likely result in loss of protein function.
Gain of Function (GOF)
No gain-of-function mutations reported.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • actin binding (GO:0003779) | • actin filament capping (GO:0051015) |
| • actin cytoskeleton organization (GO:0030036) | • plasma membrane (GO:0005886) |
Pathways
• Regulation of actin cytoskeleton (KEGG: hsa04810)
• Signaling by Rho GTPases (Reactome: R-HSA-194315)
Protein Summary
The CRACD protein is a 1,084-amino-acid polypeptide that localizes to the plasma membrane and interacts with actin capping proteins. It inhibits capping activity, allowing continued actin polymerization. This function is critical for dynamic cytoskeletal rearrangements during cell migration and adhesion.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CRACD Knockout HEK293 Cell Line | EDJ-KQ12236 | Human | 57482 | Details Get a Quote |
| CRACDL Knockout HEK293 Cell Line | EDJ-KQ12998 | Human | 343990 | Details Get a Quote |
| CRACD Knockout HCT 116 Cell Line | EDJ-KQ42245 | Human | 57482 | Details Get a Quote |
| CRACD Knockout HeLa Cell Line | EDJ-KQ42246 | Human | 57482 | Details Get a Quote |
| CRACDL Knockout A-549 Cell Line | EDJ-KQ42247 | Human | 343990 | Details Get a Quote |
| CRACDL Knockout HCT 116 Cell Line | EDJ-KQ42248 | Human | 343990 | Details Get a Quote |
| CRACDL Knockout HeLa Cell Line | EDJ-KQ42249 | Human | 343990 | Details Get a Quote |
| CRACD Knockout A-549 Cell Line | EDJ-KQ65359 | Human | 57482 | Details Get a Quote |
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