CHST8: Carbohydrate Sulfotransferase 8
Gene encoding a Golgi enzyme involved in proteoglycan sulfation and neuroendocrine function
Gene Information Card
| Symbol | CHST8 |
|---|---|
| Full Name | Carbohydrate Sulfotransferase 8 |
| Gene Type | protein-coding |
| Chromosomal Location | 19q13.11 |
| NCBI Gene ID | 64377 ncbi.nlm.nih.gov/gene/64377 |
| Ensembl ID | ENSG00000104879 |
| UniProt ID | Q9H2A9 |
| OMIM ID | 610191 |
| HGNC ID | 19325 |
| Aliases | GALNAC4ST1, GalNAc4-ST1, MGC138207 |
Description
CHST8 encodes a Golgi membrane-bound sulfotransferase that catalyzes the transfer of sulfate to the C4 hydroxyl of N-acetylgalactosamine (GalNAc) residues on chondroitin sulfate and dermatan sulfate. This enzyme is critical for the biosynthesis of sulfated proteoglycans, influencing cell signaling, extracellular matrix organization, and neuroendocrine function. CHST8 is highly expressed in the pituitary gland and brain.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Pituitary adenoma | Altered CHST8 expression affects sulfation of glycoproteins, potentially influencing hormone secretion and tumor growth | PMID: 23468642 |
| Colorectal cancer | CHST8 downregulation correlates with poor prognosis; loss of sulfation may promote tumor invasion | PMID: 25695632 |
| Breast cancer | CHST8 methylation and reduced expression associated with aggressive subtypes | PMID: 27562873 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Pituitary gland | 48.2 | High |
| Cerebral cortex | 12.5 | Medium |
| Cerebellum | 8.9 | Medium |
| Adrenal gland | 6.1 | Low |
| Liver | 1.3 | Not detected |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HeLa | 5.4 | Cervical cancer cell line |
| SH-SY5Y | 12.1 | Neuroblastoma cell line |
| MCF7 | 2.8 | Breast cancer cell line |
| HepG2 | 1.1 | Hepatocellular carcinoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.1015C>T (p.Arg339Trp) | Missense | <0.01% | Unknown functional effect; rare population variant |
| c.1246G>A (p.Gly416Ser) | Missense | <0.01% | Predicted damaging by in silico tools |
Mutation functional classification
Loss of Function (LOF)
No confirmed loss-of-function mutations reported in ClinVar or COSMIC.
Gain of Function (GOF)
No gain-of-function mutations described.
Dominant Negative (DN)
No dominant-negative mutations reported.
View complete mutation data：
Gene Ontology (GO)
| • N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity (GO:0001517) | • Golgi apparatus (GO:0005794) |
| • peptide sulfation (GO:0018149) | • glycosaminoglycan metabolic process (GO:0030203) |
| • chondroitin sulfate biosynthetic process (GO:0030206) |
Pathways
• Chondroitin sulfate / dermatan sulfate biosynthesis (Reactome: R-HSA-1793185)
• Glycosaminoglycan metabolism (KEGG: hsa00532)
Protein Summary
CHST8 is a type II transmembrane protein localized to the Golgi apparatus. It contains a sulfotransferase domain that transfers sulfate from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to GalNAc residues. The enzyme is essential for the formation of chondroitin sulfate and dermatan sulfate chains, which are key components of proteoglycans involved in cell adhesion, migration, and signaling. CHST8 expression is particularly high in the pituitary, suggesting a role in neuroendocrine regulation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| CHST8 Knockout HEK293 Cell Line | EDJ-KQ12919 | Human | 64377 | Details Get a Quote |
| CHST8 Knockout HeLa Cell Line | EDJ-KQ42124 | Human | 64377 | Details Get a Quote |
| CHST8 Knockout A-549 Cell Line | EDJ-KQ65561 | Human | 64377 | Details Get a Quote |
| CHST8 Knockout HCT 116 Cell Line | EDJ-KQ73991 | Human | 64377 | Details Get a Quote |
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