ARHGAP35
Rho GTPase Activating Protein 35
Gene Information Card
| Symbol | ARHGAP35 |
|---|---|
| Full Name | Rho GTPase Activating Protein 35 |
| Gene Type | Protein coding |
| Chromosomal Location | 19q13.32 |
| NCBI Gene ID | 2909 ncbi.nlm.nih.gov/gene/2909 |
| Ensembl ID | ENSG00000160007 |
| UniProt ID | Q9NRY4 |
| OMIM ID | 605277 |
| HGNC ID | 676 |
| Aliases | p190RhoGAP, GRF-1, GRLF1, p190-A, p190A |
Description
ARHGAP35 encodes a member of the Rho GTPase-activating protein (RhoGAP) family. The protein, p190RhoGAP, stimulates the intrinsic GTPase activity of Rho family GTPases (RhoA, Rac1, Cdc42), converting them to the inactive GDP-bound state. It functions as a tumor suppressor by negatively regulating Rho-mediated signaling, cell migration, and proliferation. ARHGAP35 is frequently mutated or deleted in various cancers.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Breast cancer | Loss of function mutations lead to increased RhoA activity, promoting invasion and metastasis. | COSMIC, ClinVar |
| Lung adenocarcinoma | Somatic mutations and deletions reduce GAP activity, enhancing tumor growth. | COSMIC, NCBI Gene |
| Colorectal cancer | Frameshift and nonsense mutations contribute to Rho pathway hyperactivation. | COSMIC, ClinVar |
| Endometrial cancer | Recurrent mutations in the RhoGAP domain impair GTP hydrolysis. | COSMIC |
| Glioblastoma | Decreased expression correlates with poor prognosis and increased cell motility. | NCBI Gene |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Brain | 12.5 | Medium |
| Lung | 8.3 | Low |
| Breast | 7.1 | Low |
| Colon | 6.9 | Low |
| Ovary | 9.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| MCF7 (breast cancer) | 10.2 | Moderate expression |
| A549 (lung cancer) | 7.5 | Low expression |
| HCT116 (colorectal cancer) | 6.8 | Low expression |
| HEK293 (embryonic kidney) | 11.0 | Moderate expression |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.2101C>T (p.Gln701*) | Nonsense | <1% in breast cancer | Truncation, loss of GAP domain |
| c.1234_1235del (p.Lys412fs) | Frameshift | <1% in colorectal cancer | Loss of function |
| c.2560G>A (p.Gly854Arg) | Missense | <1% in lung adenocarcinoma | Impaired GTPase activation |
| c.1789C>T (p.Arg597*) | Nonsense | <1% in endometrial cancer | Premature stop, loss of function |
Mutation functional classification
Loss of Function (LOF)
Nonsense and frameshift mutations that truncate the protein, particularly within the RhoGAP domain, result in loss of GTPase-activating activity and tumor suppressor function.
Gain of Function (GOF)
No gain-of-function mutations have been reported for ARHGAP35.
Dominant Negative (DN)
No dominant-negative mutations have been characterized for ARHGAP35.
View complete mutation data：
Gene Ontology (GO)
| • GTPase activator activity | • Rho GTPase binding |
| • regulation of cell migration | • negative regulation of cell proliferation |
| • actin cytoskeleton organization |
Pathways
• Rho GTPase cycle
• Signaling by Rho family GTPases
• Regulation of actin dynamics for phagocytic cup formation
Protein Summary
ARHGAP35 encodes p190RhoGAP, a 190 kDa protein containing an N-terminal GTPase-binding domain and a C-terminal RhoGAP domain. It inactivates RhoA, Rac1, and Cdc42 by accelerating GTP hydrolysis. The protein localizes to the cytoplasm and focal adhesions, regulating cytoskeletal dynamics, cell adhesion, and migration. Loss of function contributes to oncogenic transformation.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| ARHGAP35 Knockout HEK293 Cell Line | EDJ-KQ4796 | Human | 2909 | Details Get a Quote |
| ARHGAP35 Knockout A-549 Cell Line | EDJ-KQ27561 | Human | 2909 | Details Get a Quote |
| ARHGAP35 Knockout HCT 116 Cell Line | EDJ-KQ27562 | Human | 2909 | Details Get a Quote |
| ARHGAP35 Knockout HeLa Cell Line | EDJ-KQ27563 | Human | 2909 | Details Get a Quote |
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